INSERTION-SEQUENCE-DEPENDENT REARRANGEMENTS OF PSEUDOMONAS-CEPACIA PLASMID PTGL1
INSERTION-SEQUENCE-DEPENDENT REARRANGEMENTS OF PSEUDOMONAS-CEPACIA PLASMID PTGL1
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DOI：
10.1128/jb.169.1.224-230.1987
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发表时间：
1987-01-01
影响因子：
3.2
通讯作者：
LESSIE, TG
中科院分区：
文献类型：
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作者：
GAFFNEY, TD;LESSIE, TG
洋葱假单胞菌249 （ATCC 17616）含有一个170千碱基（kb）的质粒，命名为pTGL1。在该质粒上鉴定出IS405、IS408和IS411三个插入序列。我们收集的各种原生营养和营养不良衍生物包含pTGL1的变体，这些变体是通过增加和删除其他元素形成的。质粒pTGL6是一种原生动物的变体，由pTGL1进化而来，通过添加三个IS401拷贝（1.3 kb）和一个IS402拷贝（1 kb）来产生pTGL5，并将两个IS401拷贝在pTGL5上重组形成pTGL6。后一事件导致IS401拷贝的丢失和另外5.4 kb质粒DNA的丢失。利用携带上述插入序列的广宿主质粒pRP1的衍生物和携带pTGL6和pTGL5质粒片段的重组质粒作为探针，确定了洋葱洋葱基因组中各种元件的重复程度。数据表明基因组重排的频率很高，这可能有助于这种细菌的非凡适应性。
Pseudomonas cepacia 249 (ATCC 17616) harbors a 170-kilobase (kb) plasmid designated pTGL1. We identified three insertion sequences, IS405, IS408 and IS411, on this plasmid. Various prototrophic and auxotrophic derivatives in our collection contained variants of pTGL1 formed by accretion and deletion of other elements. Plasmid pTGL6, the variant in one prototroph, evolved from pTGL1 by the addition of three copies of IS401 (1.3 kb) and one of IS402 (1 kb), to generate pTGL5, and recombination between two of the copies of IS401 on pTGL5 to form pTGL6. The latter event entailed loss of one copy of IS401 and an additional 5.4 kb of plasmid DNA. Derivatives of the broad-host-range plasmid pRP1 carrying the above insertion sequences and recombinant plasmids carrying fragments of plasmids pTGL6 and pTGL5 were used as probes to ascertain the extent of reiteration of the various elements in the P. cepacia genome. The data indicate a high frequency of genomic rearrangements which presumably contributes to the extraordinary adaptability of this bacterium.