Multilayered Lipid Membrane Stacks for Biocatalysis Using Membrane Enzymes
Multilayered Lipid Membrane Stacks for Biocatalysis Using Membrane Enzymes
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DOI：
10.1002/adfm.201606265
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发表时间：
2017-05-04
影响因子：
19
通讯作者：
Jeuken, Lars J. C.
中科院分区：
文献类型：
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作者：
Heath, George R.;Li, Mengqiu;Jeuken, Lars J. C.
多层或堆叠的脂质膜是生物学中的常见原理，并且与单脂质膜相比具有各种功能优势，例如它们在空间上组织过程、区室化分子以及大大增加表面积并因此增加膜蛋白浓度的能力。在这里，报道了一种多层脂质膜系统的超分子组装，其中聚-L-赖氨酸静电连接带负电荷的脂质膜。当掺入合适的膜酶时，无论是泛醇氧化酶（来自大肠杆菌的细胞色素bo（3））还是耐氧氢化酶（来自真养罗尔斯通氏菌的膜结合氢化酶），循环伏安法（CV）显示生物催化活性随着每个额外的膜层线性增加。酶和电极之间的电子转移是由存在于脂质相中的醌池介导的。使用原子力显微镜，CV，和荧光显微镜，它推断，醌能够通过缺陷位点的脂质膜相互连接的堆叠的脂质膜层之间的扩散。这种组装类似于相互连接的类囊体膜或线粒体的折叠层，并且在生物技术应用中具有巨大的模仿潜力，例如能源生产或生物传感。
Multilayered or stacked lipid membranes are a common principle in biology and have various functional advantages compared to single-lipid membranes, such as their ability to spatially organize processes, compartmentalize molecules, and greatly increase surface area and hence membrane protein concentration. Here, a supramolecular assembly of a multilayered lipid membrane system is reported in which poly-l-lysine electrostatically links negatively charged lipid membranes. When suitable membrane enzymes are incorporated, either an ubiquinol oxidase (cytochrome bo(3) from Escherichia coli) or an oxygen tolerant hydrogenase (the membrane-bound hydrogenase from Ralstonia eutropha), cyclic voltammetry (CV) reveals a linear increase in bio-catalytic activity with each additional membrane layer. Electron transfer between the enzymes and the electrode is mediated by the quinone pool that is present in the lipid phase. Using atomic force microscopy, CV, and fluorescence microscopy it is deduced that quinones are able to diffuse between the stacked lipid membrane layers via defect sites where the lipid membranes are inter-connected. This assembly is akin to that of interconnected thylakoid membranes or the folded lamella of mitochondria and has significant potential for mimicry in biotechnology applications such as energy production or biosensing.