Effects of Fis on Escherichia coli gene expression during different growth stages
Effects of Fis on Escherichia coli gene expression during different growth stages
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DOI：
10.1099/mic.0.2007/008565-0
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发表时间：
2007-09-01
期刊：
影响因子：
2.8
通讯作者：
Osuna, Robert
中科院分区：
文献类型：
--
作者：
Bradley, Meranda D.;Beach, Michael B.;Osuna, Robert
Fis是大肠杆菌中的一种核相关蛋白，在丰富培养基中的早期指数生长期间丰富，但在稳定期供应不足。它作为转录调节因子的作用已被证明是越来越多的基因。为了深入了解Fis在丰富培养基中生长的不同阶段对大肠杆菌基因表达的全局影响，在早期、中期、晚期指数生长期和稳定生长期期间在Fis和野生型菌株中进行DNA微阵列分析。结果发现了231个显著调控的基因，分布在15个功能类别中。在所有的生长阶段检查观察到的监管效果。在不同的生长阶段，观察到一些参与翻译，鞭毛生物合成和运动，营养运输，碳化合物代谢和能量代谢的基因的协调上调。还观察到参与应激反应、氨基酸和核苷酸生物合成、能量和中间代谢以及营养物质运输的基因的协调下调。随着细胞从早期指数生长期过渡到晚期指数生长期，不同功能类别的基因受到调节，并且逐渐转向大部分下调。结果表明，生长阶段依赖性的Fis表达触发协调调节的15类功能相关的基因在特定阶段的大肠杆菌培养物的生长。
Fis is a nucleoid-associated protein in Escherichia coli that is abundant during early exponential growth in rich medium but is in short supply during stationary phase. Its role as a transcriptional regulator has been demonstrated for an increasing number of genes. In order to gain insight into the global effects of Fis on E coli gene expression during different stages of growth in rich medium, DNA microarray analyses were conducted in fis and wild-type strains during early, mid-, late-exponential and stationary growth phases. The results uncovered 231 significantly regulated genes that were distributed over 15 functional categories. Regulatory effects were observed at all growth stages examined. Coordinate upregulation was observed for a number of genes involved in translation, flagellar biosynthesis and motility, nutrient transport, carbon compound metabolism, and energy metabolism at different growth stages. Coordinate down-regulation was also observed for genes involved in stress response, amino acid and nucleotide biosynthesis, energy and intermediary metabolism, and nutrient transport. As cells transitioned from the early to the late-exponential growth phase, different functional categories of genes were regulated, and a gradual shift occurred towards mostly down-regulation. The results demonstrate that the growth phase-dependent Fis expression triggers coordinate regulation of 15 categories of functionally related genes during specific stages of growth of an E coli culture.