Short-chain fatty acids stimulate colonic transit via intraluminal 5-HT release in rats
Short-chain fatty acids stimulate colonic transit via intraluminal 5-HT release in rats
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DOI：
10.1152/ajpregu.00442.2002
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发表时间：
2003-05-01
影响因子：
2.8
通讯作者：
Takahashi, T
中科院分区：
文献类型：
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作者：
Fukumoto, S;Tatewaki, M;Takahashi, T
我们研究了短链脂肪酸（SCFAs）的生理浓度是否会影响清醒大鼠的结肠转运和结肠运动。向近端结肠腔内给予SCFAs（100 - 200 mM）可显著加速结肠转运。辣椒素颈迷走神经周围处理、阿托品、六甲铵以及迷走神经切断术可消除SCFAs对结肠转运的刺激作用，但胍乙啶则不能。利多卡因和5 - 羟色胺（HT）（3）受体拮抗剂进行腔内预处理也可消除SCFAs对结肠转运的刺激作用。向腔内给予SCFAs会引起近端结肠收缩，并向中结肠和远端结肠传播。SCFAs可使体外血管隔离且腔内灌注的大鼠结肠的5 - 羟色胺腔内浓度显著增加。这表明，肠嗜铬细胞响应SCFAs释放5 - 羟色胺，刺激位于迷走神经感觉纤维上的5 - 羟色胺3受体。感觉信息传递至迷走神经传出纤维，刺激结肠肌间神经丛释放乙酰胆碱，从而导致肌肉收缩。
We studied whether physiological concentration of short-chain fatty acids (SCFAs) affects colonic transit and colonic motility in conscious rats. Intraluminal administration of SCFAs (100-200 mM) into the proximal colon significantly accelerated colonic transit. The stimulatory effect of SCFAs on colonic transit was abolished by perivagal capsaicin treatment, atropine, hexamethonium, and vagotomy, but not by guanethidine. The stimulatory effect of SCFAs on colonic transit was also abolished by intraluminal pretreatment with lidocaine and a 5-hydroxytryptamine (HT)(3) receptor antagonist. Intraluminal administration of SCFAs provoked contractions at the proximal colon, which migrated to the mid- and distal colon. SCFAs caused a significant increase in the luminal concentration of 5-HT of the vascularly isolated and luminally perfused rat colon ex vivo. It is suggested that the release of 5-HT from enterochromaffin cells in response to SCFAs stimulates 5-HT3 receptors located on the vagal sensory fibers. The sensory information is transferred to the vagal efferent and stimulates the release of acetylcholine from the colonic myenteric plexus, resulting in muscle contraction.