REGULATION OF CAPSULAR POLYSACCHARIDE SYNTHESIS IN ESCHERICHIA-COLI K-12 - CHARACTERIZATION OF 3 REGULATORY GENES
REGULATION OF CAPSULAR POLYSACCHARIDE SYNTHESIS IN ESCHERICHIA-COLI K-12 - CHARACTERIZATION OF 3 REGULATORY GENES
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DOI：
10.1128/jb.162.3.1111-1119.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
TORRESCABASSA, A
中科院分区：
文献类型：
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作者：
GOTTESMAN, S;TRISLER, P;TORRESCABASSA, A
大肠杆菌荚膜多糖的合成随生长培养基、温度和遗传背景而变化。与荚膜合成（cps）所必需的基因的lac融合表明，这些基因在体内受到lon基因产物的负调控。本文报道了3个新的调控基因（rcs，即荚膜合成调控基因）突变的分离、特性鉴定以及图谱位置，这些基因控制着相同融合基因的表达。rcsA和rcsB是荚膜合成的正调控因子，rcsA位于大肠杆菌图谱的43分钟处，而rcsB位于47分钟处。rcsC是荚膜合成的负调控因子，位于47分钟处，靠近rcsB。所有3个调控突变都与结构基因cpsA - F或lon无连锁关系。所有3个rcs基因的突变对野生型均为隐性。据推测，lon可能通过调控其中一个正调控因子的可利用性来间接调控荚膜合成。
The synthesis of the E. coli capsular polysaccharide varies with growth medium and temperature and genetic background. lac fusions to genes necessary for capsule synthesis (cps) demonstrated that these genes are regulated negatively in vivo by the lon gene product. The isolation, characterization, and map positions of mutations of 3 new regulatory genes (rcs, for regulator of capsule synthesis) that control expression of these same fusions are reported here. rcsA and rcsB are positive regulators of capsule synthesis, rcsA is located at min 43 on the E. coli map, whereas rcsB lies at 47 min. rcsC, a negative regulator of capsule synthesis, is located at min 47, close to rcsB. All 3 regulatory mutations are unlinked to either the structural genes cpsA-F or lon. Mutations in all 3 rcs genes are recessive to the wild type. It is postulated that lon may regulate capsule synthesis indirectly, by regulating the availability of 1 of the positive regulators.