Molecular Responses of Maize Shoot to a Plant Derived Smoke Solution
Molecular Responses of Maize Shoot to a Plant Derived Smoke Solution
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DOI：
10.3390/ijms20061319
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发表时间：
2019-03-15
影响因子：
5.6
通讯作者：
Komatsu, Setsuko
中科院分区：
文献类型：
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作者：
Aslam, Muhammad Mudasar;Rehman, Shafiq;Komatsu, Setsuko
植物产生的烟雾对植物生长有影响。利用无凝胶/无标记蛋白质组学技术，研究了植物烟雾对玉米生长的分子机制。植物烟雾能增加玉米的根长和地上部长度。蛋白质组学分析表明，2000ppm植物烟雾改变了4日龄玉米新梢中69种蛋白质的丰度。植物产生的烟雾改变了细胞质、叶绿体和细胞膜中的蛋白质。催化、信号和核苷酸结合蛋白发生了变化。与蔗糖合成酶、核苷酸、信号转导和谷胱甘肽相关的蛋白质显著增加，而与细胞壁、脂类、光合作用和氨基酸降解相关的蛋白质减少。基于蛋白质组学和免疫印迹分析，玉米茎中的核酮糖-1，5-二磷酸羧基/加氧酶(Rubisco)活性降低，而植物烟雾对Rubisco激活酶活性没有影响。抗坏血酸过氧化物酶不受影响；然而，植物来源的烟雾降低了过氧化氢还蛋白。此外，酶活性和mRNA表达分析的结果证实了抗坏血酸过氧化物酶和过氧化还蛋白清除系统的调节。这些结果表明，蔗糖合成酶、核苷酸、信号转导和谷胱甘肽相关蛋白的增加，以及植物烟雾对活性氧及其清除系统的调节，可能会促进玉米的生长。
Plant-derived smoke has effects on plant growth. To find the molecular mechanism of plant-derived smoke on maize, a gel-free/label-free proteomic technique was used. The length of root and shoot were increased in maize by plant-derived smoke. Proteomic analysis revealed that 2000 ppm plant-derived smoke changed the abundance of 69 proteins in 4-days old maize shoot. Proteins in cytoplasm, chloroplast, and cell membrane were altered by plant-derived smoke. Catalytic, signaling, and nucleotide binding proteins were changed. Proteins related to sucrose synthase, nucleotides, signaling, and glutathione were significantly increased; however, cell wall, lipids, photosynthetic, and amino acid degradations related proteins were decreased. Based on proteomic and immunoblot analyses, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) was decreased; however, RuBisCO activase was not changed by plant-derived smoke in maize shoot. Ascorbate peroxidase was not affected; however, peroxiredoxin was decreased by plant-derived smoke. Furthermore, the results from enzyme-activity and mRNA-expression analyses confirmed regulation of ascorbate peroxidase and the peroxiredoxinin reactive oxygen scavenging system. These results suggest that increases in sucrose synthase, nucleotides, signaling, and glutathione related proteins combined with regulation of reactive oxygen species and their scavenging system in response to plant-derived smoke may improve maize growth.