Diversification of the Caenorhabditis heat shock response by Helitron transposable elements
Diversification of the Caenorhabditis heat shock response by Helitron transposable elements
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DOI：
10.7554/elife.51139
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发表时间：
2019-12-11
期刊：
影响因子：
7.7
通讯作者：
Pasquinelli, Amy E.
中科院分区：
文献类型：
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作者：
Garrigues, Jacob M.;Tsu, Brian V.;Pasquinelli, Amy E.
热休克因子1（HSF-1）是热休克反应（HSR）的关键调节因子。在热休克时，HSF-1结合被称为热休克元件（HSEs）的保守基序，并驱动在这种应激期间对细胞保护重要的基因的表达。值得注意的是，我们发现在多个小杆线虫物种中大量的HSE存在于Helitrons（一种DNA转座子）中。与Helitron包埋的HSEs的功能一致，在热休克后，它们显示出增加的HSF-1和RNA聚合酶II占据率以及C.优雅有趣的是，我们发现不同的基因似乎被纳入到HSR的物种特异性Helitron插入在C。和秀丽隐杆线虫C. briggsae和菌株特异性插入之间的不同野生型C.优雅我们的研究揭示了以前未识别的HSF-1的目标，并表明Helitron插入负责重新布线和多样化的小杆线虫HSR。
Heat Shock Factor 1 (HSF-1) is a key regulator of the heat shock response (HSR). Upon heat shock, HSF-1 binds well-conserved motifs, called Heat Shock Elements (HSEs), and drives expression of genes important for cellular protection during this stress. Remarkably, we found that substantial numbers of HSEs in multiple Caenorhabditis species reside within Helitrons, a type of DNA transposon. Consistent with Helitron-embedded HSEs being functional, upon heat shock they display increased HSF-1 and RNA polymerase II occupancy and up-regulation of nearby genes in C. elegans. Interestingly, we found that different genes appear to be incorporated into the HSR by species-specific Helitron insertions in C. elegans and C. briggsae and by strain-specific insertions among different wild isolates of C. elegans. Our studies uncover previously unidentified targets of HSF-1 and show that Helitron insertions are responsible for rewiring and diversifying the Caenorhabditis HSR.