A short method for the isolation of somatotrophs from the rat pituitary gland.
A short method for the isolation of somatotrophs from the rat pituitary gland.
复制标题
从大鼠垂体中分离生长激素的一种简短方法。
DOI：
10.1210/endo-96-3-792
复制
发表时间：
1975
期刊：
影响因子：
4.8
通讯作者：
W. Hymer
中科院分区：
文献类型：
--
作者：
G. Snyder;W. Hymer
描述了应用密度梯度离心程序从分散的大鼠垂体前叶细胞中分离生长激素。使用牛血清白蛋白的两个连续不连续梯度来富集生长激素细胞群，从初始细胞悬浮液中的频率 38% 到最终纯度 85%。分离的生长激素细胞在整个分离过程中保持组织化学和超微结构的完整性。当与 6 mM 二丁酰 3',5'-环 AMP 一起孵育时，分离的生长激素细胞还会释放可免疫测定的生长激素。该方法具有简单、简短的优点。
Application of a density gradient centrifugation procedure for the isolation of somatotrophs from dispersed rat anterior pituitary cells is described. Two sequential discontinuous gradients of bovine serum albumin are used to enrich the somatotroph population from a frequency of 38% in the initial cell suspension to a final purity of 85%. The isolated somatotrophs retain both histochemical and ultrastructural integrity throughout the isolation procedure. The isolated somatotrophs also release immunoassayable growth hormone when incubated with 6 mM dibutyryl 3',5'-cyclic AMP. The method offers the advantages of being both simple and short.