Biotinylated bi- and tetra-antennary glycoconjugates for Escherichia coli detection.
Biotinylated bi- and tetra-antennary glycoconjugates for Escherichia coli detection.
复制标题
用于大肠杆菌检测的生物素化双触角和四触角糖缀合物。
DOI：
10.1002/cbic.200800188
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Iyer,SuriS
中科院分区：
文献类型：
--
作者：
Hatch,DuaneM;Weiss,AlisonA;Kale,RameshR;Iyer,SuriS
Glycans cover the surface of all mammalian cells. Several toxins and pathogens use these glycans to bind and infect the cell. Using a versatile modular synthetic strategy, we have developed biotinylated bi‐ and tetraantennary glycoconjugates to capture and detectE. coliand compared the capturing ability of these molecules to commercial polyclonal antibodies. Magnetic beads were coated with biotinylated glycoconjugate or antibody, and these beads were used to capture, isolate, and quantify bacterial recovery by using a luminescence assay. The glycoconjugate‐coated magnetic beads outperformed antibody‐coated magnetic beads in sensitivity and selectivity when compared under identical experimental conditions. Glycoconjugates could captureEscherichia colifrom stagnant water, and the ability of a panel of glycoconjugates to capture a selection of pathogenic bacteria was also evaluated. To the best of our knowledge, this study represents the first comprehensive study that compares synthetic glycoconjugates and antibodies forE. colidetection. The glycoconjugates are also very stable and inexpensive. The results presented here are expected to lead to an increased interest in developing glycoconjugate‐based high affinity reagents for diagnostics.
