Interleukin-6 directly influences proliferation and invasion potential of head and neck cancer cells
Interleukin-6 directly influences proliferation and invasion potential of head and neck cancer cells
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DOI：
10.1007/s00405-007-0264-6
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发表时间：
2007-07-01
影响因子：
2.6
通讯作者：
Noda, Yutaka
中科院分区：
文献类型：
--
作者：
Kanazawa, Takeharu;Nishino, Hiroshi;Noda, Yutaka
白细胞介素-6 （IL-6）是免疫反应和造血的多功能调节剂。近年来，有研究报道IL-6的表达与多种癌症患者的预后相关。在这项研究中，我们研究了IL-6是否影响头颈部鳞状细胞癌（HNSCCs）的增殖和侵袭潜能。所有HNSCC细胞系HEp-2、HSC-2、HSC-4和SAS均通过逆转录聚合酶链反应（RT-PCR）检测表达IL-6受体（IL-6R）和负责信号转导的糖蛋白130。HEp-2、HSC-2和HSC-4也产生IL-6。IL-6对HSC-2和SAS的增殖有抑制作用，但对所有细胞系的侵袭能力均有增强作用。此外，IL-6下调可溶性IL-6R的表达。抗il - 6r抗体消除了IL-6诱导的增殖抑制和侵袭增加。IL-6刺激还能诱导细胞外调节的蛋白激酶1/2活化和血管内皮生长因子释放增加。这些结果表明，IL-6作为肿瘤转移的第一步，可以直接影响细胞增殖和侵袭潜能。
Interleukin-6 (IL-6) is a multifunctional regulator of immune response and hematopoiesis. Recently, it has been reported that expression of IL-6 is correlated with prognosis in various cancer patients. In this study, we investigated whether the proliferation and invasion potential of head and neck squamous cell carcinomas (HNSCCs) were influenced by IL-6. All HNSCC cell lines, HEp-2, HSC-2, HSC-4, and SAS, were tested by reverse transcription-polymerase chain reaction (RT-PCR) and expressed the IL-6 receptor (IL-6R), and glycoprotein 130, which is responsible for signal transduction. HEp-2, HSC-2, and HSC-4 also produced IL-6. IL-6 inhibited the proliferation of HSC-2 and SAS, but the invasion potential of all the cell lines increased. Moreover, IL-6 down-regulated soluble IL-6R expression. Anti-IL-6R antibody abrogated the inhibited proliferation and increased invasion induced by IL-6. IL-6 stimulation also induced the extracellular regulated protein kinase 1/2 activation and increased vascular endothelial growth factor release. These results suggest that IL-6 can directly influence cell proliferation and the invasion potential as the first step of tumor metastasis.