Three-dimensional direct cell patterning in collagen hydrogels with near-infrared femtosecond laser.
Three-dimensional direct cell patterning in collagen hydrogels with near-infrared femtosecond laser.
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DOI：
10.1038/srep17203
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发表时间：
2015-11-25
影响因子：
4.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Hribar KC;Meggs K;Liu J;Zhu W;Qu X;Chen S
我们报告了一种在原位水凝胶中进行三维（3D）细胞图案化的方法。包封细胞的胶原蛋白水凝胶内的金纳米棒吸收聚焦的近红外飞秒激光束，使胶原蛋白局部变性并形成通道，细胞在其中迁移，增殖并在3D中对齐。重要的是，图案分辨率可根据写入速度和激光功率进行调节，并且使用更高的写入速度和更低的激光强度实现高细胞活力（>90%）。总的来说，这种图案化技术提供了一种灵活的直接写入方法，该方法适用于3D对准至关重要的组织工程系统（例如血管、神经、心脏和肌肉组织）。
We report a methodology for three-dimensional (3D) cell patterning in a hydrogel in situ. Gold nanorods within a cell-encapsulating collagen hydrogel absorb a focused near-infrared femtosecond laser beam, locally denaturing the collagen and forming channels, into which cells migrate, proliferate, and align in 3D. Importantly, pattern resolution is tunable based on writing speed and laser power, and high cell viability (>90%) is achieved using higher writing speeds and lower laser intensities. Overall, this patterning technique presents a flexible direct-write method that is applicable in tissue engineering systems where 3D alignment is critical (such as vascular, neural, cardiac, and muscle tissue).