Glial Cell Markers in the Reeler Mutant Mouse: A Biochemical and Immunohistological Study
Glial Cell Markers in the Reeler Mutant Mouse: A Biochemical and Immunohistological Study
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Reeler 突变小鼠中的胶质细胞标记：生化和免疫组织学研究
DOI：
10.1111/j.1471-4159.1981.tb02395.x
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发表时间：
1981
影响因子：
4.7
通讯作者：
O. Langley
中科院分区：
文献类型：
--
作者：
M. S. Ghalidour;P. Derer;G. Labourdette;J. Delaunoy;O. Langley
用生化方法测定了该突变小鼠小脑和大脑中S LOO蛋白、2‘3’-环磷酸腺苷、3‘-磷酸水解酶、碳酸酐酶同工酶II和丁酰胆碱酯酶的含量。这项研究显示，星形胶质细胞和少突胶质细胞含有异常数量的这些成分。小脑颗粒分数和大脑颗粒分数中钙离子浓度显著升高(分别为50%和%)。其比活力显著提高，小脑提高120%，大脑提高40%。相反，卷曲小脑中的Sloo蛋白浓度降低了40%，大脑中降低了25%，而卷曲小脑中的CNP比活性增加了30%。此外，应用免疫组织化学技术，对S 100蛋白、胶质纤维酸性蛋白和CAII进行免疫组织化学染色，研究了胶质细胞的分布。显然，神经胶质细胞的密度没有受到明显影响。然而，高尔基体上皮细胞通常位置不正常，其Bergmann纤维发育较差。
The glial cell contents of S lOO protein, 2′3′‐cyclic AMP, 3′‐ phosphohydrolase (CNP), isoenzyme II of carbonic anhydrase (CAII) and butyrylcholinesterase (BuChE) were biochemically determined in the cerebellum and cerebrum of the reeler mutant mouse. Astrocytes and oligodendrocytes, shown by this study, contain abnormal amounts of these components. The CAII concentration was significantly increased in the particulate fraction of the reeler cerebellum and cerebrum (by 50% and 89%, respectively). The BuChE specific activity was greatly increased in the reeler, by 120% for cerebellum and by 40% in cerebrum. In contrast, the SlOO protein concentration was reduced in the reeler cerebellum by 40% and by 25% in cerebrum, while the CNP specific activity increased by 30% in the reeler cerebellum. In addition, the glial cell distribution was studied by immunohistological techniques with antibodies directed against S 100 protein, glial fibrillary acidic protein (GFA) and CAII. Apparently the density of glial cells is not significantly affected. However, the Golgi epithelial cells were usually abnormally placed and their Bergmann fibres were less well developed.