2D metal azolate framework as nanozyme for amperometric detection of glucose at physiological pH and alkaline medium
2D metal azolate framework as nanozyme for amperometric detection of glucose at physiological pH and alkaline medium
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DOI：
10.1007/s00604-021-04737-w
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发表时间：
2021-02-10
影响因子：
5.7
通讯作者：
Rahman, Md Mahbubur
中科院分区：
文献类型：
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作者：
Adeel, Muhammad;Canzonieri, Vincenzo;Rahman, Md Mahbubur
采用简单的水热法合成了钴基二维金属唑类化合物骨架纳米片（MAF-5-Co-II NS）。产物与MAF-5（Zn）同构。制备的MAF-5-Co-II NS具有高的比表面积（1155 m2/g）、纯度和结晶度。MAF-5-Co-II NS修饰的丝网印刷电极（MAF-5-Co-II NS/SPE）用于用磷酸盐缓冲盐水（PBS，pH 7.4）和NaOH（0.1 M，pH 13.0）溶液稀释的人血浆（BP）样品中葡萄糖的非酶检测。MAF-5-Co-II NS纳米酶在中性和碱性条件下均表现出良好的氧化还原活性，并形成Co-II/Co-III氧化还原对，催化氧化葡萄糖。在优化的检测电位下，该传感器对PBS稀释的葡萄糖溶液中两个较宽浓度范围内的葡萄糖氧化均呈现计时电流响应（62.80至180 μ M和305至8055 μ M）和NaOH稀释的（58.90至117.6 μ M和180至10，055 μ M）BP样品，其在糖尿病患者餐前（4.4- 7.2mM）和餐后（10 mM）的血糖水平限度内（由美国糖尿病协会推荐）。传感器的检测限为ca。0.25和0.05 μ M，最大灵敏度为ca.在PBS和NaOH稀释的BP样品中，分别为36.55和1361.65 mA/cm（2）/mM。由于MAF-5-Co-II NS中疏水连接基（2-乙基咪唑）的存在，该传感器在中性和碱性介质中也显示出优异的稳定性，良好的重复性和再现性，以及无干扰的信号。因此，MAF-5-Co-II NS是一种很有前途的纳米酶，用于开发用于人体体液中葡萄糖检测的一次性传感器。
The synthesis of Co-based two-dimensional (2D) metal azolate framework nanosheets (MAF-5-Co-II NS) is described using a simple hydrothermal method. The product was isostructural to MAF-5 (Zn). The as-prepared MAF-5-Co-II NS exhibited high surface area (1155 m(2)/g), purity, and crystallinity. The MAF-5-Co-II NS-modified screen-printed electrode (MAF-5-Co-II NS/SPE) was used for nonenzymatic detection of glucose in diluted human blood plasma (BP) samples with phosphate buffer saline (PBS, pH 7.4) and NaOH (0.1 M, pH 13.0) solutions. The MAF-5-Co-II NS nanozyme displayed good redox activity in both neutral and alkaline media with the formation of Co-II/Co-III redox pair, which induced the catalytic oxidation of glucose. Under the optimized detection potential, the sensor presented a chronoamperometric current response for the oxidation of glucose with two wide concentration ranges in PBS-diluted (62.80 to 180 mu M and 305 to 8055 mu M) and NaOH-diluted (58.90 to 117.6 mu M and 180 to 10,055 mu M) BP samples, which were within the limit of blood glucose levels of diabetic patients before (4.4-7.2 mM) and after (10 mM) meals (recommended by the American Diabetes Association). The sensor has a limit of detection of ca. 0.25 and 0.05 mu M, respectively, and maximum sensitivity of ca. 36.55 and 1361.65 mA/cm(2)/mM, respectively, in PBS- and NaOH-diluted BP samples. The sensor also displayed excellent stability in the neutral and alkaline media due to the existence of hydrophobic linkers (2-ethyl imidazole) in the MAF-5-Co-II NS, good repeatability and reproducibility, and interference-free signals. Thus, MAF-5-Co-II NS is a promising nanozyme for the development of the disposable type of sensor for glucose detection in human body fluids.