NUCLEOTIDE-SEQUENCE OF HUMAN MONOCYTE INTERLEUKIN-1 PRECURSOR CDNA
NUCLEOTIDE-SEQUENCE OF HUMAN MONOCYTE INTERLEUKIN-1 PRECURSOR CDNA
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DOI：
10.1073/pnas.81.24.7907
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
DINARELLO, CA
中科院分区：
文献类型：
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作者：
AURON, PE;WEBB, AC;DINARELLO, CA
白介素1(IL-1)是一种具有多种生物活性的调节宿主防御和免疫反应的蛋白质。本文报道了人IL-1c[互补]DNA的分离。它编码269个氨基酸(30,747 mW)的前体多肽。通过与该基因杂交获得的mRNA在无网织红细胞系统中翻译，产生可免疫沉淀的IL-1。将这种杂交选择的mRNA注射到非洲爪哇卵母细胞中，非洲爪哇卵母细胞随后分泌具有生物活性的IL-1。该核苷酸序列表明，IL-1最初被翻译为前体分子，随后被加工成通常与IL-1活性相关的15,000-20,000 mW蛋白质。
Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses. The isolation of human IL-1 c[complementary]DNA is reported. It encodes a precursor polypeptide of 269 amino acids (30,747 MW). mRNA isolated by hybridization to this cDNA was translated in a reticulocyte cell-free system, yielding immunoprecipitable IL-1. This hybrid-selected mRNA was injected into Xenopus laevis oocytes, which subsequently secreted biologically active IL-1. The cDNA nucleotide sequence suggests that IL-1 is initially translated as a precursor molecule that is subsequently processed into the 15,000-20,000 MW protein usually associated with IL-1 activity.