Characterization of the functional binding properties of antibody conjugated quantum dots
Characterization of the functional binding properties of antibody conjugated quantum dots
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DOI：
10.1021/nl062706i
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发表时间：
2007-07-01
期刊：
影响因子：
10.8
通讯作者：
Silva, Gabriel A.
中科院分区：
文献类型：
--
作者：
Pathak, Smita;Davidson, Marie C.;Silva, Gabriel A.
抗体结合量子点是一种新兴的生物系统高分辨率标记技术。在这项工作中，我们确定了功能性抗体的数量（即，在空间上可用于功能性结合靶蛋白的抗体）与半导体量子点缀合。这对于用这些方法标记的生物数据的解释至关重要。我们发现，可用的功能性抗体的数量对于不同的缀合方法变化显著，并且低于先前估计的。这些结果可能为改进生物制剂的量子点标记提供潜在的策略。
Antibody conjugated quantum dots are an emerging technology for high-resolution labeling of biological systems. In this work we determined the number of functional antibodies (i.e., antibodies that are sterically available for functional binding to target proteins) conjugated to semiconductor quantum dots. This is critical for the interpretation of biological data labeled with these methods. We found that the number of available functional antibodies varied significantly for different conjugation methods and are lower than previously estimated. These results may suggest potential strategies for improving quantum dot labeling of biological preparations.