High fiber dietary and sodium butyrate attenuate experimental autoimmune hepatitis through regulation of immune regulatory cells and intestinal barrier
High fiber dietary and sodium butyrate attenuate experimental autoimmune hepatitis through regulation of immune regulatory cells and intestinal barrier
复制标题
高纤维饮食和丁酸钠通过调节免疫调节细胞和肠道屏障减轻实验性自身免疫性肝炎
DOI：
10.1016/j.cellimm.2018.03.003
复制
发表时间：
2018-06-01
影响因子：
4.3
通讯作者：
Chen, Yong-Ping
中科院分区：
文献类型：
--
作者：
Hu, En-De;Chen, Da-Zhi;Chen, Yong-Ping
自身免疫性肝炎（AIH）是伴随Treg/Th17失衡和肠道通透性增高的慢性自身免疫性肝病。我们研究了高纤维饮食和丁酸钠对实验性自身免疫性肝炎患者Treg/Th17和肠道屏障功能的影响。采用肝抗原（S100）腹腔注射诱导实验性自身免疫性肝炎小鼠模型，将小鼠分为正常对照组、S100模型对照组、S100 +高纤维饮食和S100 +丁酸钠。检测血清转氨酶和肝脏组织学。采用高效液相色谱法测定粪便中的短链脂肪酸。流式细胞术检测CD4 + C25 + Foxp3 + Treg和CD4 + IL-17 + Th17的比值。紧密连接蛋白（Zonula ocluden）、Occludin和Claudin-1被用于评估肠道屏障功能，肝脏中的大肠杆菌蛋白也被用于评估肠道屏障功能。与模型对照组相比，高纤维饲粮和丁酸钠均显著降低了小鼠的血清转氨酶水平和肝损伤程度。高纤维饲粮和丁酸钠组小鼠Treg/Th17比值显著高于模型对照组。此外，高纤维饲粮和丁酸钠显著提高了肠道紧密连接蛋白水平，降低了肝脏中大肠杆菌蛋白水平。综上所述，高纤维饮食和丁酸钠可通过调节免疫调节细胞和肠道屏障功能来减轻自身免疫性肝炎的发展。
Autoimmune hepatitis (AIH) is chronic autoimmune liver disease accompanied with the imbalance of Treg/Th17 and increased intestinal permeability. We investigated the effects of a high fiber diet and sodium butyrate on the Treg/Th17 and intestinal barrier function in an experimental autoimmune hepatitis. Intraperitoneal injection of hepatic antigen (S100) was used to induce experimental autoimmune hepatitis mice model and mice were divided into normal control, S100 model control, S100 plus high fiber diet and S100 plus sodium butyrate. Serum aminotransferases and liver histology were examined. Short chain fatty acids in feces were determined by HPLC. The ratio of CD4 + C25 + Foxp3 + Treg and CD4 + IL-17 + Th17 were evaluated by flow cytometry. Tight junction proteins Zonula ocluden, Occludin and Claudin-1 were used to assess intestinal barrier function, so does Escherichia coli protein in the liver. Mice fed with either high fiber diet or sodium butyrate showed significantly lower levers of serum aminotransferases and minor liver injury compared to that of model control. Moreover, the ratio of Treg/Th17 was significantly higher in high fiber diet and sodium butyrate fed mice than that in model control. Furthermore, high fiber diet and sodium butyrate significantly increased intestinal tight junction proteins and decreased Escherichia Coll protein in the liver. In conclusion, high fiber diet and sodium butyrate can attenuate development of autoimmune hepatitis through regulation of immune regulatory cells and intestinal barrier function.