Electrophysiological and morphological properties of light and dark cells isolated from mudpuppy taste buds.
Electrophysiological and morphological properties of light and dark cells isolated from mudpuppy taste buds.
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从泥巴犬味蕾中分离出的亮细胞和暗细胞的电生理学和形态学特性。
DOI：
10.1002/cne.903460411
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Kinnamon,JC
中科院分区：
文献类型：
--
作者：
McPheeters,M;Barber,AJ;Kinnamon,SC;Kinnamon,JC
分离的Necturustaste受体细胞通过Giga-seal全细胞记录和电子显微镜进行研究，以将电生理特性与味觉细胞结构特征相关联。通过在细胞质的核上和顶端区域中存在致密颗粒包来鉴定暗（I型）细胞。响应于一系列来自−80 mV保持电位的去极化电压命令，这些细胞表现出短暂的TTX敏感性内向Na+电流、持续的外向K+电流和缓慢失活的内向Ca++电流。光（II型）细胞被确定为缺乏颗粒包和丰富的滑面内质网分布在整个细胞。此外，孤立的轻细胞有明确的泡状包涵体的细胞质和细胞膜上的水泡。根据电生理学标准，将光细胞分为两个功能群：具有内向和外向电流的细胞和仅具有外向电流的细胞。具有内向和外向电流的亮细胞具有电压激活的Na+、K+和Ca++电流，其性质与暗细胞相似。相比之下，第二组光细胞只有电压激活外向K+电流响应去极化电压命令。这些数据表明，具有内向和外向电流的暗细胞和亮细胞能够产生动作电位并释放神经递质到味觉传入神经元上以响应味觉刺激。相比之下，具有外向电流的光细胞只可能在味蕾中发挥不同的功能。© 1994 Wiley利斯公司
IsolatedNecturustaste receptor cells were studied by giga‐seal whole‐cell recording and electron microscopy to correlate electrophysiological properties with taste cell structural features. Dark (type I) cells were identified by the presence of dense granular packets in the supranuclear and apical regions of the cytoplasm. In response to a series of depolarizing voltage commands from a holding potential of −80 mV, these cells exhibited a transient, TTX‐sensitive inward Na+current, a sustained outward K+current, and a slowly inactivating inward Ca++current. Light (type II) cells were identified by a lack of granular packets and by an abundance of smooth endoplasmic reticulum distributed throughout the cell. In addition, isolated light cells had clear vesicular inclusions in the cytoplasm and blebs on the plasma membrane. Light cells were divided into two functional populations based upon electrophysiological criteria: cells with inward and outward currents, and cells with outward currents only. Light cells with inward and outward currents had voltage‐activated Na+, K+, and Ca++currents with properties similar to those of dark cells. In contrast, the second group of light cells had only voltageactivated outward K+currents in response to depolarizing voltage commands. These data suggest that dark cells and light cells with inward and outward currents are capable of generating action potentials and releasing neurotransmitters onto gustatory afferent neurons in response to taste stimulation. In contrast, light cells with outward currents only likely serve a different function in the taste bud. © 1994 Wiley‐Liss, Inc.