Genotoxic effects of asbestos in humans
Genotoxic effects of asbestos in humans
复制标题
DOI：
10.1016/j.mrfmmm.2004.06.027
复制
发表时间：
2004-09-03
影响因子：
2.3
通讯作者：
Kyrtopoulos, S
中科院分区：
文献类型：
--
作者：
Dusinská, M;Collins, A;Kyrtopoulos, S
由于石棉纤维在建筑材料和其他产品中的持久性，石棉致癌和非致癌影响的风险继续存在。因此，仍然需要对石棉和矿物纤维的毒性作用进行流行病学和机理研究。目前的分子流行病学研究是在斯洛伐克的一家前石棉水泥厂进行的。共调查了82名受试者，61名暴露受试者（24名吸烟者和37名非吸烟者），21名工厂对照（8名吸烟者和13名非吸烟者）。工人们接触石棉的时间在5到40年之间。虽然在过去40年中接触石棉的程度相对较高，但在取样时，生产车间的石棉浓度仅超过斯洛伐克的职业限值（0.001纤维/立方厘米）3至5倍。办公面积水平低于这一限制。测量了暴露、效应和个体易感性的生物标志物，包括DNA损伤（使用彗星试验测定链断裂[SB]、碱基氧化和烷基化）;细胞遗传学参数;和个体DNA修复能力（使用改良彗星试验测定8-氧代鸟嘌呤处的切口）。氧化嘧啶显着高于暴露的男性相比，非暴露（P = 0.04）。S Bs（P = 0.04）与年龄呈正相关，DNA烷基化损伤（P= 0.04）与年龄呈正相关。此外，氧化嘧啶（P= 0.01）和烷基化碱（P = 0.001）与职业暴露年数密切相关。暴露组和对照组受试者的微核率无差异。修复能力总体上没有显示出任何暴露的影响，尽管女性对照组的切口率高于女性暴露受试者。石棉作业工人染色体畸变率显著高于对照组（P = 0.01）。这一发现与已知的染色体畸变与癌症风险的相关性一致。(C)2004年由Elsevier B. V.出版
Risks of carcinogenic and non-carcinogenic effects from asbestos continue owing to the persistence of the fibres in building materials and other products. For this reason, epidemiological and mechanistic research on the toxic effects of asbestos and mineral fibres is still needed. The present molecular epidemiological study was conducted in a former asbestos cement plant in Slovakia. Altogether 82 subjects were investigated, 61 exposed subjects (24 smokers and 37 nor-smokers), and 21 factory controls (8 smokers and 13 non-smokers). Workers were exposed to asbestos for between 5 and 40 years. Though the exposure to asbestos during past 40 years was relatively high, at the time of sampling the concentrations of asbestos in the production hall exceeded the Slovak occupational limit (0.001 fibre/cm(3)) by a factor of only 3-5. The office area levels were below this limit. Biomarkers of exposure, effect and individual susceptibility were measured, including DNA damage (strand breaks [SBs], base oxidation and alkylation, using the comet assay); cytogenctic parameters; and individual DNA repair capacity (incision at 8-oxoguanine measured using a modified comet assay). Oxidised pyrimidines were significantly higher in exposed men compared with non-exposed (P = 0.04). There was also a positive association between S Bs (P = 0.04) and age, and alkylation damage to DNA(P= 0.04) and age. Moreover, oxidised pyrimidines (P= 0.01) and alkylated bases (P = 0.001) strongly correlated with years of occupational exposure. Micronucleus frequency did not differ between exposed and control subjects. Repair capacity overall did not show any effect of exposure, though female controls had higher incision rates than did female exposed subjects. However, exposed asbestos workers had significantly higher numbers of chromosomal aberrations (P = 0.01) compared with control group. This finding is consistent with the known association of chromosome aberrations with cancer-risk. (C) 2004 Published by Elsevier B.V.