Capillary electrophoretic separations of peptides using micelle-forming compounds and cyclodextrins as additives.
Capillary electrophoretic separations of peptides using micelle-forming compounds and cyclodextrins as additives.
复制标题
使用胶束形成化合物和环糊精作为添加剂对肽进行毛细管电泳分离。
DOI：
10.1016/0021-9673(90)85147-n
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Novotny,M
中科院分区：
文献类型：
--
作者：
Liu,J;Cobb,KA;Novotny,M
证明了电动毛细管色谱分离结构相似的模型肽和胰蛋白酶的价值。描述了模型肽在含有十二烷基三甲基溴化铵、十六烷基三甲基溴化铵、十二烷基硫酸钠和两种环糊精作为添加剂的缓冲体系中的行为。在不同的分析环境下，这些添加剂对具有相似净电荷但不同疏水性的肽表现出一定的有益效果。分离未衍生化的肽，利用紫外检测，提出。此外，还对邻苯二甲醛、荧光胺和新试剂3-（4-羧基苯甲酰基）-2-喹啉甲醛衍生化肽荧光产物的分离进行了论证和讨论。还注意到环糊精的有益光谱检测效果。
The value of electrokinetic capillary chromatography for separating structurally similar model peptides and tryptic digests is demonstrated. The behavior of model peptides in buffer systems containing dodecyltrimethylammonium bromide, hexadecyltrimethylammonium bromide, sodium dodecyl sulfate and two cyclodextrins as additives is described. These additives, under different analytical circumstances, exhibit certain beneficial effects for peptides with similar net charges but different hydrophobicities. Separations of underivatized peptides, utilizing UV detection, are presented. In addition, separation of fluorescent products of peptides derivatized witho-phthalaldehyde, fluorescamine, and a new reagent, 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde, are demonstrated and discussed. Beneficial spectroscopic detection effects with cyclodextrin are also noted.