Immunohistochemical expression analysis of the human interferon-inducible gene IFI16, a member of the HIN200 family, not restricted to hematopoietic cells
Immunohistochemical expression analysis of the human interferon-inducible gene IFI16, a member of the HIN200 family, not restricted to hematopoietic cells
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DOI：
10.1089/107999002320271413
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发表时间：
2002-07-01
影响因子：
2.3
通讯作者：
Landolfo, S
中科院分区：
文献类型：
--
作者：
Gariglio, M;Azzimonti, B;Landolfo, S
这是第一次描述的干扰素（IFN）诱导基因IFI 16在正常组织中表达的广泛免疫组化分析。通过使用针对其C-末端片段的多克隆抗体，在Western印迹中识别其三种紧密迁移的同种型，实现了对石蜡包埋组织中IFI 16的免疫组织化学检测。结果清楚地表明，IFI 16表达不限于造血区室。在正常成人组织中，它在复层鳞状上皮中很突出，在增殖区室的副基底层细胞中特别强烈，但在更分化的基底上层中逐渐减少。了解IFI 16在体内的表达是至关重要的解释从体外研究中获得的结果和阐明其生理作用。IFI 16在正常人组织中的组成型表达和更广泛的分布，不限于造血区室，强烈支持在细胞分化中发挥重要作用的可能性，所述细胞分化可以通过其他刺激物（例如IFN）进一步调节。
This is the first description of an extensive immunohistochemical analysis of interferon (IFN)-inducible gene IFI16 expression in normal tissues. Immunohistochemical detection of IFI16 in paraffin-embedded tissues is achieved by using a polyclonal antibody raised against its C-terminal fragment that recognizes its three closely migrating isoforms in Western blotting. The results clearly indicate that IFI16 expression is not restricted to the hematopoietic compartment. In normal adult human tissues, it is prominent in stratified squamous epithelia and particularly intense in parabasal cells in the proliferating compartments, but it gradually decreases in the more differentiated suprabasal layers. Understanding of IFI16 expression in vivo is essential for interpretation of the results obtained from in vitro studies and elucidation of its physiologic role. The constitutive expression and wider distribution of IFI16 in normal human tissues, not restricted to the hematopoietic compartment, strongly support the possibility of an important role in cell differentiation that can be further modulated by other stimuli, such as IFN.