Identification of quantitative trait loci across recombinant inbred lines and testcross populations for traits of agronomic importance in rice
Identification of quantitative trait loci across recombinant inbred lines and testcross populations for traits of agronomic importance in rice
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DOI：
10.1534/genetics.105.047209
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发表时间：
2006-02-01
期刊：
影响因子：
3.3
通讯作者：
He, GC
中科院分区：
文献类型：
--
作者：
You, AQ;Lu, XG;He, GC
本研究旨在确定在重组自交系（RIL）中检测到的控制重要农艺性状的数量性状位点（QTL）是否也在水稻测交（TC）杂种中表达。利用B5与中国最广泛种植的杂交水稻品种明恢63杂交的RIL群体构建了一张遗传图谱。以水稻细胞质雄性不育系和核不育系培矮64s为材料，用3个保持系与重组自交系杂交，获得4个TC杂种群体。4个TC群体中7个性状的RIL值与F1代的RIL值均呈显著相关。共检测到27个主效QTL。其中，在两个或更多的TC群体中检测到对RIL中的7个性状中的每一个具有最强效应的QTL，并且在一个TC群体中检测到另外6个QTL。上位性分析表明，影响。上位性QTL相对较弱，具有杂交组合特异性。通过对水稻QTL数据的检索，发现在不同环境下，效应最强的QTL在大范围的群体中具有位置收敛性。由于主效QTL在不同的测验种间、不同的遗传背景和环境中表达，因此它是基因操作和进一步应用于水稻育种的有价值的目标。当培育出表达主效QTL的恢复系时，可用于许多杂交组合。
This studs was conducted to determine whether quantitative trait loci (QTL) controlling traits of agronomic importance detected in recombinant inbred lines (RILs) are also expressed in testcross (TC) hybrids of rice. A genetic map was constructed using an RIL Population derived from a cross between B5 and Minghui 63, a parent of the most widely grown hybrid rice cultivar in China. Four TC hybrid populations were produced by crossing the RILs with three maintaining lines for the widely used cytoplasmic male-sterile (CMS) lines and the genic male-sterile line Peiai64s. The mean values of the RILs for the seven traits investigated were significantly correlated to those of the F-1 hybrids in the four TC populations. Twenty-seven main-effect QTL were identified in the RILs. Of these, the QTL that had the strongest effect on each of the seven traits in the RILs was detected in two or more of the TC Populations, and six other QTL were detected in one TC population. Epistatic analysis revealed that the effect. of epistatic QTL was relatively weak and cross combination specific. Searching publicly available QTL data in rice revealed the positional convergence of the QTL with the strongest effect in a wide range Of Populations and under different environments. Since the main-effect QTL is expressed across different testers, and in different genetic backgrounds and environments, it is a valuable target for gene manipulation and for further application in rice breeding. When a restorer line that expresses main-effect QTL is bred, it Could be used in a number of cross combinations.