Fission yeast Slp1: An effector of the Mad2-dependent spindle checkpoint
Fission yeast Slp1: An effector of the Mad2-dependent spindle checkpoint
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DOI：
10.1126/science.279.5353.1045
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发表时间：
1998-02-13
期刊：
影响因子：
56.9
通讯作者：
Matsumoto, T
中科院分区：
文献类型：
--
作者：
Kim, SH;Lin, DP;Matsumoto, T
MAD2是纺锤体检查点的一个组成部分，它推迟后期的开始，直到所有的染色体都附着在纺锤体上。MAD2与SLp1形成复合体，SLp1是一种WD(色氨酸-天冬氨酸)重复蛋白，对后期的开始是必不可少的。当两个蛋白质之间的物理相互作用被破坏时，纺锤体检查点不再起作用。后期事件，如染色体解聚和下一轮DNA复制，即使在纺锤体组装不完整的情况下也没有延迟。这种依赖的缓解似乎是后期促进复合体介导的泛素依赖的蛋白分解放松调控的结果。
Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.