TECHNICAL PITFALLS LEADING TO ERRORS IN QUANTITATION OF PLASMA AMINO ACIDS
TECHNICAL PITFALLS LEADING TO ERRORS IN QUANTITATION OF PLASMA AMINO ACIDS
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DOI：
10.1016/0009-8981(69)90226-5
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发表时间：
1969-01-01
影响因子：
5
通讯作者：
HANSEN, S
中科院分区：
文献类型：
--
作者：
PERRY, TL;HANSEN, S
本文描述了用于氨基酸分析的血浆制备和自动氨基酸分析仪操作中看似微不足道的变化，这些变化可能导致牛磺酸、磷酸乙醇胺、二羧酸氨基酸、谷氨酰胺、二硫化物氨基酸和色氨酸的定量测定出现重大误差。这些技术陷阱很容易避免。由此产生的血浆氨基酸测量准确性的提高对于某些与正常差异细微的代谢或营养失调的有意义的研究可能至关重要。
This paper describes seemingly trivial variations in the preparation of blood plasma for amino acid analysis, and in the operation of the automatic amino acid analyzer, that can lead to major errors in the quantitative determination of taurine, phosphoethanolamine, the dicarboxylic amino acids, glutamine, the disulphide amino acids, and tryptophan. These technical pitfalls can easily be avoided. The resulting improved accuracy in measurement of plasma amino acids may be essential for meaningful studies of some metabolic or nutritional disorders where differences from normal are subtle.