Immature platelet fraction for prediction of platelet engraftment after allogeneic stem cell transplantation
Immature platelet fraction for prediction of platelet engraftment after allogeneic stem cell transplantation
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DOI：
10.1038/sj.bmt.1705623
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发表时间：
2007-04-01
影响因子：
4.8
通讯作者：
Asakura, H.
中科院分区：
文献类型：
--
作者：
Takami, A.;Shibayama, M.;Asakura, H.
血小板再生是同种异体干细胞移植植入过程中一个重要且独立的因素。使用血细胞计数器的全自动低细胞计数现在可以实现可靠的定量。网织血小板的阳离子，表示为未成熟血小板分数（IPF）。我们研究了 6 名接受同种异体外周血干细胞移植 (PBSCT) 的患者、12 名接受骨髓移植 (BMT) 的患者和 7 名接受脐带血移植 (CBT) 的患者的 IPF 动力学。预处理治疗导致三系造血功能立即迅速下降。在 PBSCT 后 11 天、BMT 后 18 天和 CBT 后 19 天的平均持续时间后，IPF 短暂上升至 3% 以上。这分别比血小板植入早 1、4 和 13 天。线性相关模型显示 IPF 的增加与移植后三系植入之间存在密切关联。因此，IPF 计数可以提供一种易于测量的血小板生成活性，从而早期评估骨髓功能并监测血小板再生。
Platelet regeneration represents an important and separate element in the engraftment process for allogeneic stem cell transplantation. Fully automated low cytometry using blood cell counters now allows reliable quanti. cation of reticulated platelets, expressed as the immature platelet fraction (IPF). We studied the kinetics of IPF in six patients grafted with allogeneic peripheral blood stem cell transplantation (PBSCT), 12 patients with bone marrow transplantation (BMT) and seven patients with cord blood transplantation (CBT). Preconditioning therapy caused an immediate and rapid fall in tri-lineage hematopoiesis. IPF rose transiently above 3% after a mean duration of 11 days post-PBSCT, 18 days post-BMT and 19 days post-CBT. This was 1, 4 and 13 days earlier than platelet engraftment, respectively. A linear correlation model showed a close association between the rise of IPF and tri-lineage engraftment after transplantation. IPF counting may thus provide an accessible measure of thrombopoietic activity, leading to early evaluation of marrow function and allowing monitoring of platelet regeneration.