Prognostic value of meiotic spindle imaging on fertilization rate and embryo development in in vitro-matured human oocytes
Prognostic value of meiotic spindle imaging on fertilization rate and embryo development in in vitro-matured human oocytes
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DOI：
10.1016/j.fertnstert.2007.06.088
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发表时间：
2008-08-01
影响因子：
6.7
通讯作者：
Borges, Edson, Jr.
中科院分区：
文献类型：
--
作者：
de Almeida Ferreira Braga, Daniela Paes;Savio Figueira, Rita de Cassia;Borges, Edson, Jr.
目的：探讨控制卵巢刺激（COS）周期中纺锤体显像与卵胞浆内单精子注射（ICSI）结果的关系。设计：前瞻性研究。地点：辅助生殖中心。患者：30例接受ICSI周期。干预措施：在ICSI前评估234例体内成熟卵和101例体外成熟卵的减数分裂纺锤体。主要观察指标：减数分裂纺锤体成像、受精率和胚胎发育。结果：74.3%的体内成熟卵母细胞和73.8%的体外成熟卵母细胞存在纺锤体。萌发囊泡和中期i期卵母细胞纺锤体检出率分别为50%和86%。体外成熟卵母细胞受精率为71.8%，受精卵母细胞中检出纺锤体的比例为75.6%，仅为34.8%。%的未受精卵母细胞。在体外成熟卵母细胞中，受精率为66.1%，81.4%的受精卵母细胞和59.1%的未受精卵母细胞检测到纺锤体。43个体外成熟衍生胚胎中有10个（23.2%）被认为是高质量的，它们都来自纺锤体检测的卵母细胞，这意味着高质量胚胎的总数增加了13.0%。结论：减数分裂纺锤体成像可用于预测体外成熟卵母细胞的发育。然而，其他因素可能导致体外成熟卵母细胞发育能力下降。
Objective: To evaluate the relationship between spindle visualization and intracytoplasmic sperm injection (ICSI) outcomes in controlled ovarian stimulation (COS) cycles.Design: Prospective study.Setting: Assisted reproduction center.Patient(s): Thirty patients undergoing ICSI cycles.Intervention(s): Meiotic spindle was evaluated before ICSI in 234 in vivo- and 101 in vitro-matured oocytesMain Outcome Measure(s): Meiotic spindle imaging, fertilization rate, and embryo development.Result(s): Spindle was present in 74.3% and 73.8% of the in vivo- and in vitro-matured oocytes, respectively. Spindle detection rate in oocytes derived from germinal vesicle and metaphase-I stage was, respectively, 50% and 86%. The fertilization rate achieved by the in vivo-matured oocytes was 71.8%, and spindle was detected in 75.6% of the fertilized oocytes and only 34.8.% of the nonfertilized oocytes. In the in vitro-matured oocytes, the fertilization rate was 66.1 %, and spindle was detected in 81.4% of the fertilized oocytes and in 59.1 % of the nonfertilized oocytes. Ten out of 43 (23.2%) in vitro-matured derived embryos were considered to be high quality, all derived from spindle-detected oocytes, which represents in increase of 13.0% on the overall number of high-quality embryos.Conclusion(s): Meiotic spindle imaging may be useful to predict in vitro-matured oocyte development. However, other factors may contribute to the decreased developmental competence of in vitro-matured oocytes.