Using cytometric bead arrays to detect cytokines in the serum of patients with different types of pulmonary tuberculosis
Using cytometric bead arrays to detect cytokines in the serum of patients with different types of pulmonary tuberculosis
复制标题
利用流式微珠芯片检测不同类型肺结核患者血清中的细胞因子
DOI：
10.1177/2058738419845176
复制
发表时间：
2019-04-23
影响因子：
3.5
通讯作者：
Bao, Fukai
中科院分区：
文献类型：
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作者：
Bai, Ruolan;Tao, Luyan;Bao, Fukai
细胞因子在介导结核病（TB）的免疫应答中起着至关重要的作用。本研究的目的是评估不同形式肺结核（PTB）患者的细胞因子水平，并确定诊断PTB的有价值的细胞因子生物标志物。我们测量了健康供者（n = 30）血清中六种细胞因子（白介素（IL-2、IL-4、IL-6和IL-10）、肿瘤坏死因子（tnf - α）和干扰素- γ (ifn - γ)）的水平。活动性肺结核患者（n = 46）和潜伏性肺结核患者（LTBI, n = 38）采用细胞头阵列检测。血清样品中六种细胞因子的水平被迅速、灵敏、同时测量。PTB组IL-2、IL-6、IL-10、ifn - γ水平均显著高于健康供者（P < 0.01或P < 0.05）。此外，与LTBI组相比，活动性PTB组IL-2、IL-6、IL-10和ifn - γ水平显著升高（P < 0.01或P < 0.05）。然而，PTB患者血清中的IL-4和tnf - α水平与健康供体组的血清中IL-4和tnf - α水平没有显着相关性。我们的数据表明，IL-2、IL-6、IL-10和ifn - γ可能在结核病的辅助诊断中有用，并可作为区分LTBI和TB的生物标志物。
Cytokines play a crucial role in mediating immune responses to tuberculosis (TB). The aim of this study was to evaluate the levels of cytokines in patients with different forms of pulmonary tuberculosis (PTB) and identify valuable cytokine biomarkers for the diagnosis of PTB. We measured the levels of six cytokines (interleukin (IL-2, IL-4, IL-6, and IL-10), tumor necrosis factor (TNF-alpha), and interferon-gamma (IFN-gamma)) in the serum of healthy donors (n = 30). Patients with active PTB (n = 46) and those with latent tuberculosis infection (LTBI, n = 38) were examined using cytometric bead arrays. The levels of the six cytokines in the serum samples were measured promptly, sensitively, and simultaneously. The levels of IL-2, IL-6, IL-10, and IFN-gamma were significantly higher in the PTB group compared with those reported in the healthy donors (P < 0.01 or P < 0.05). In addition, significantly higher levels of IL-2, IL-6, IL-10, and IFN-gamma were found in the active PTB group compared with those observed in the LTBI group (P < 0.01 or P < 0.05). However, the levels of IL-4 and TNF-alpha in the sera of patients from the PTB group did not show a significant correlation with those measured in the healthy donor group. Our data demonstrated that IL-2, IL-6, IL-10, and IFN-gamma may be useful in the auxiliary diagnosis of tuberculosis and as biomarkers for distinguishing LTBI from TB.