Muscle Fiber Differentiation and Growth Patterns During Hyperplasia and Hypertrophy in the Ricefield Eel Regulated by Myogenic Regulatory Factors
Muscle Fiber Differentiation and Growth Patterns During Hyperplasia and Hypertrophy in the Ricefield Eel Regulated by Myogenic Regulatory Factors
复制标题
生肌调节因子调控稻田鳗（Monopterus albus）增生和肥大过程中的肌纤维分化和生长模式。
DOI：
10.1002/naaq.10025
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发表时间：
2018-04-01
影响因子：
1
通讯作者：
Liu, Li
中科院分区：
文献类型：
--
作者：
Chu, Wuying;Chen, Dunxue;Liu, Li
肌肉生长主要通过两个过程实现，即增生（纤维数量增加）和肥大（纤维尺寸增加），并且由肌源性调节因子（MRF）家族调节。以体重5 ~ 180 g的黄鳝（Monopterus albus）白色肌肉为材料，研究了4种MRF（Myf 5、MRF 4、MyoD和myogenin [MyoG]）的肌纤维组成和表达模式。结果表明，随着体质量从5 ~ 114 g的增加，肌纤维平均直径逐渐增大。5-45 g鳗鱼肌肉中直径<20 m的小肌纤维比例最高，64-104 g鳗鱼肌肉中直径在20 ~ 60 m的肌纤维比例最高，114-180 g鳗鱼肌肉中直径在60 m以上的肌纤维比例最高。Myf 5和MyoD的表达在以中小肌纤维为主的阶段持续增加。MRF 4和MyoG在小肌纤维比例高的阶段表达较低，但在中等肌纤维比例高的阶段表达水平升高。四种MRF的表达水平对肌肉增生或肥大的过程具有调节作用。结果表明，黄鳝的早期发育以增生为主，而肥大发生在发育后期。本研究结果为黄鳝肌肉发育提供了有价值的知识。
Muscle growth is mainly achieved by two processes, hyperplasia (fiber number increase) and hypertrophy (fiber size increase), and is regulated by the myogenic regulatory factor (MRF) family. The muscle fiber composition and expressional patterns of the four MRFs (Myf5, MRF4, MyoD, and myogenin [MyoG]) were characterized in the white muscle of Ricefield Eels Monopterus albus ranging from 5 to 180g. Results showed that the mean muscle fiber diameters gradually increased as body mass increased from 5 to 114g. The small muscle fibers (diameter < 20m) were found in the highest ratio in 5-45-g eels; most of the fibers ranged from 20 to 60m for 64-104-g fish; and muscle mainly consisted of fibers over 60m in 114-180-g fish. The expression of Myf5 and MyoD increased continuously at the stages mainly consisting of the small and medium muscle fibers. Expression of MRF4 and MyoG was low at the stages with high ratios of small muscle fibers, but their expression levels increased at the stages with high ratios of medium muscle fibers. The expression levels of the four MRFs exhibited a regulatory effect on the process of muscle hyperplasia or hypertrophy. The data indicated that hyperplasia was the major event at the earlier stages of the Ricefield Eel, whereas hypertrophy occurred at later developmental stages. The results of our study provide valuable knowledge regarding muscle development in Ricefield Eels.