STUDIES ON BATCH AND CONTINUOUS CULTURES OF BOTRYOCOCCUS-BRAUNII - HYDROCARBON PRODUCTION IN RELATION TO PHYSIOLOGICAL-STATE, CELL ULTRASTRUCTURE, AND PHOSPHATE NUTRITION
STUDIES ON BATCH AND CONTINUOUS CULTURES OF BOTRYOCOCCUS-BRAUNII - HYDROCARBON PRODUCTION IN RELATION TO PHYSIOLOGICAL-STATE, CELL ULTRASTRUCTURE, AND PHOSPHATE NUTRITION
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DOI：
10.1002/bit.260270312
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发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
DESANTI, O
中科院分区：
文献类型：
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作者：
CASADEVALL, E;DIF, D;DESANTI, O
采用间歇培养和连续培养的方法，研究了富烃褐藻在气举条件下的生长情况。在分批培养和不同稀释率的连续培养中，布劳尼布氏杆菌的生理状态发生了很大的变化。研究了这些变化对碳氢化合物（性质、相对丰度、位置、水平、生产力）和胞外多糖生产的可能影响。讨论了布氏贝氏菌的生理状态与其产烃和多糖的关系，并与一般单细胞藻类进行了比较。考虑了导致高生产力阶段向低生产力阶段过渡的因素。为此，首先检查了细胞超微结构的变化以及在批培养过程中发生的变性。随后测定了培养基中一些参数（pH、磷酸盐水平）的平行变化，并讨论了它们与布氏酵母生长和产烃的可能关系。最后探讨了布劳尼布氏杆菌中磷营养的主要特征及其对烃类的影响。
The growth of the hydrocarbon-rich alga B. braunii was studied under air-lift conditions using batch and continuous cultures. Large variations in the physiological state of B. braunii were achieved in batch cultures and in continuous cultures with various dilution rates. The possible effects of these variations upon hydrocarbons (nature, relative abundance, location, level, productivity) and also in the production of exocellular polysaccharides were examined. The relationships between the physiological state of B. braunii and its hydrocarbon and polysaccharide production were discussed and compared with those generally observed in unicellular algae. The factors giving rise to the transition from high to low productivity stages were considered. To this end the variations in cell ultrastructure and the resulting degeneration occurring during batch cultures were first examined. Afterward the parallel changes in some parameters of the medium (pH, phosphate level) were determined and their possible relationships with B. braunii growth and hydrocarbon production were discussed. The main features of phosphate nutrition in B. braunii and its effects on hydrocarbons were finally examined.