Structure and level of genetic diversity in various bean types evidenced with microsatellite markers isolated from a genomic enriched library
Structure and level of genetic diversity in various bean types evidenced with microsatellite markers isolated from a genomic enriched library
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DOI：
10.1007/s00122-002-0901-9
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发表时间：
2002-06-01
影响因子：
5.4
通讯作者：
Peltier, D
中科院分区：
文献类型：
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作者：
Métais, I;Hamon, B;Peltier, D
我们构建了富含微卫星基序（ATA）、（CA）、（CAC）和（GA）的菜豆基因组文库。筛选后，从富集（ATA）重复序列的文库中选择的60%的克隆含有微卫星，而从富集（GA）、（CA）和（CAC）重复序列的文库中选择的21%的克隆含有微卫星。已经开发了15对引物，允许扩增SSR位点。我们已经评估了这些位点的遗传多样性之间的45个不同的豆类品系属于9个不同的质量类型。在15个微卫星座位上共检测到81个等位基因，平均每个座位5.3个等位基因。我们已经调查了等位基因大小多态性的起源在基因座PvATA 20，其中重复的数量范围从24到85。我们已经将这些重复数的巨大差异与重复DNA区域之间的不平等交换联系起来。多样性分析显示，根据豆类型的变异性对比水平。极优良菜豆的遗传力水平较低，说明育种家的强化选择效应。
We have constructed a common bean genomic library enriched for microsatellite motifs (ATA), (CA), (CAC) and (GA). After screening, 60% of the clones selected from the library enriched for the (ATA) repeat contained microsatellites versus 21% of the clones from the library enriched for (GA) (CA) and (CAC) repeats. Fifteen primer pairs have been developed allowing for the amplification of SSR loci. We have evaluated the genetic diversity of these loci between 45 different bean lines belonging to nine various quality types. A total of 81 alleles were detected at the 15 microsatellite loci with an average of 5.3 alleles per locus. We have investigated the origin of allelic size polymorphism at the locus PvATA20 in which the number of repeats ranges from 24 to 85. We have related these large differences in repeat number to unequal crossing-over between repeated DNA regions. The diversity analysis revealed contrasted levels of variability according to the bean type. The lower level was evidenced for the very fine French bean, showing the effect of breeders intensive selection.