Role of hydrophobicity in adhesion of the dissimilatory Fe(III)-reducing bacterium Shewanella alga to amorphous Fe(III) oxide
Role of hydrophobicity in adhesion of the dissimilatory Fe(III)-reducing bacterium Shewanella alga to amorphous Fe(III) oxide
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DOI：
10.1128/aem.63.10.3837-3843.1997
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发表时间：
1997-10-01
影响因子：
4.4
通讯作者：
Nielsen, PH
中科院分区：
文献类型：
--
作者：
Caccavo, F;Schamberger, PC;Nielsen, PH
通过比较分析S，S-BrBrY和该物种的一个粘附缺陷菌株S，研究了异化Fe（LII）还原菌希瓦氏菌（Shewanella）粘附于无定形Fe（III）氧化物的机制。RAD 20，约100%的S。HPLC-BrY细胞典型地粘附于无定形Fe（III）氧化物，而少于50%的S.大量化学分析、等电点分析、飞行时间二次离子质谱和电子能谱化学分析表明，S.结果表明，S，SRAD 20细胞主要是胞外多糖，SRAD 20细胞的疏水性比S，SRAD 20细胞强。该研究代表了异化Fe（III）还原细菌对无定形Fe（III）氧化物的粘附的第一次定量分析，并且结果共同表明疏水相互作用是控制该细菌对无定形Fe（III）氧化物的粘附的因素。HNORAD 20以与S，HNOBrY相同的速率还原Fe（III）氧化物。这一结果与以前的研究结果相反，通过证明不可逆的细胞粘附是不必要的微生物还原无定形Fe（III）氧化物，这些结果表明，异化Fe（III）还原细菌和无定形Fe（III）氧化物之间的相互作用比以前认为的更复杂。
The mechanisms by which the dissimilatory Fe(LII)-reducing bacterium Shewanella alga adheres to amorphous Fe(III) oxide were examined through comparative analysis of S, alga BrY and an adhesion-deficient strain of this species, S. alga RAD20, Approximately 100% of S. alga BrY cells typically adhered to amorphous Fe(III) oxide, while less than 50% of S. alga RAD20 cells adhered, Bulk chemical analysis, isoelectric point analysis, and cell surface analysis by time-of-flight secondary-ion mass spectrometry and electron spectroscopy for chemical analysis demonstrated that the surfaces of S, alga BrY cells were predominantly protein but that the surfaces of S. alga RAD20 cells were predominantly exopolysaccharide, Physicochemical analyses and hydrophobic interaction assays demonstrated that S, alga BrY cells were more hydrophobic than S. alga RAD20 cells, This study represents the first quantitative analysis of the adhesion of a dissimilatory Fe(III)-reducing bacterium to amorphous Fe(III) oxide, and the results collectively suggest that hydrophobic interactions are a factor in controlling the adhesion of this bacterium to amorphous Fe(III) oxide, Despite having a reduced ability to adhere, S, alga RAD20 reduced Fe(III) oxide at a rate identical to that of S, alga BrY. This result contrasts with results of previous studies by demonstrating that irreversible cell adhesion is not requisite for microbial reduction of amorphous Fe(III) oxide, These results suggest that the interaction between dissimilatory Fe(III)-reducing bacteria and amorphous Fe(III) oxide is more complex than previously believed.