Complete genome sequence of an isolate of papaya leaf distortion mosaic virus from commercialized PRSV-resistant transgenic papaya in China.

Complete genome sequence of an isolate of papaya leaf distortion mosaic virus from commercialized PRSV-resistant transgenic papaya in China.
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DOI:
10.4149/av_2013_04_452
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发表时间:
2013
期刊:
影响因子:
1.7
通讯作者:
D. Tuo;W. Shen;P. Yan;Ch Li;L. Gao;X. Li;H. Li;P. Zhou
D. Tuo;W. Shen;P. Yan;Ch Li;L. Gao;X. Li;H. Li;P. Zhou
中科院分区:
医学4区
文献类型:
--
作者:
D. Tuo;W. Shen;P. Yan;Ch Li;L. Gao;X. Li;H. Li;P. Zhou

文献摘要

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番木瓜叶片扭曲花叶病毒对番木瓜商品化生产具有极强的破坏性。本文测定了番木瓜叶片扭曲花叶病毒分离物的全基因组序列,命名为PLDMV-DF,侵染了中国生产的转基因番木瓜。除3‘-聚(A)尾外,该序列与来自台湾和日本的几个PLDMV分离物有很高的序列同源性,在系统发育上与来自日本的分离物关系最近。PLDMV-DF在转基因抗PRSV番木瓜中的感染可能预示着这种疾病在基因工程植物中的出现。已报道的该分离物的序列可能有助于产生抗PRSV和PLDMV的双转基因番木瓜。
Papaya leaf distortion mosaic virus is highly destructive to commercial papaya production. Here, the complete genome sequence was determined for an isolate of papaya leaf distortion mosaic virus, designated PLDMV-DF, infecting the commercialized papaya ringspot virus (PRSV)-resistant transgenic papaya from China. Excluding the 3'-poly (A) tail, the sequence shares high sequence identity to several PLDMV isolates from Taiwan and Japan and is phylogenetically most closely related to the isolate from Japan. Infection of PLDMV-DF in transgenic PRSV-resistant papaya may indicate emergence of this disease in genetically engineered plants. The reported sequence for this isolate may help generate bi-transgenic papaya resistant to PRSV and PLDMV.