Additive effects of plant expressed double-stranded RNAs on root-knot nematode development
Additive effects of plant expressed double-stranded RNAs on root-knot nematode development
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DOI：
10.1016/j.ijpara.2010.01.003
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发表时间：
2010-06-01
影响因子：
4
通讯作者：
McPherson, Michael J.
中科院分区：
文献类型：
--
作者：
Charlton, Wayne L.;Harel, H. Yael Meller;McPherson, Michael J.
异位表达的双链rna （dsRNAs）最近被证明可以抑制植物中Meloidogyne的寄生成功。我们从根结线虫中定位了两个基因；一种双氧化酶基因，与发育中的角质层和信号肽酶亚基的酪氨酸交联有关，信号肽酶是加工分泌蛋白质所需的蛋白质复合物虽然这些基因参与线虫发育的不同方面，但RNA干扰（RNAi）的表型后果是相似的，>=根中线虫数量减少50%，并且对产卵的囊状雌性发育迟缓。观察到加工后的dsRNA表达，但没有证据表明在转基因植物中发现可检测水平的小干扰rna （sirna）。据我们所知，我们首次表明，通过杂交适当的拟南芥系来结合这些dsRNAs的表达会产生一种加性效应，进一步减少线虫的数量和发育能力。结合RNAi靶基因有可能提高RNAi的功效，并可能控制不同种类或属的线虫在感兴趣的作物。(C) 2010年澳大利亚寄生虫学学会Elsevier Ltd.出版。版权所有。
Ectopically expressed double-stranded RNAs (dsRNAs) have recently been shown to suppress parasitic success of Meloidogyne spp. in plants. We have targeted two genes from the root-knot nematode Meloidogyne incognita; a dual oxidase gene implicated in the tyrosine cross-linking of the developing cuticle and a subunit of signal peptidase, a protein complex required for the processing of secreted proteins. While these genes are involved in different aspects of nematode development, the phenotypic consequences of RNA interference (RNAi) were similar with, >= 50% reduction in nematode numbers in the roots and retardation of development to the egg-producing saccate females. Expression of processed dsRNA was observed, but no evidence of detectable levels of small interfering RNAs (siRNAs) was found in the transgenic plants. We show, to our knowledge for the first time, that combining expression of these dsRNAs by crossing appropriate Arabidopsis thaliana lines resulted in an additive effect that further reduced nematode numbers and developmental capacity. Combining RNAi target genes has the potential to enhance the efficacy of RNAi and may allow control of different nematode species or genera in the crop of interest. (C) 2010 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.