A putative angiogenin receptor in angiogenin-responsive human endothelial cells
A putative angiogenin receptor in angiogenin-responsive human endothelial cells
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DOI：
10.1073/pnas.94.6.2204
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发表时间：
1997-03-18
影响因子：
11.1
通讯作者：
Vallee, BL
中科院分区：
文献类型：
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作者：
Hu, GF;Riordan, JF;Vallee, BL
血管生成素刺激稀疏培养物中人内皮细胞的[H-3]胸苷掺入和增殖。在这些条件下，可以检测到特异性结合血管生成素的170-kDa细胞表面蛋白。血管生成素刺激的细胞生长是浓度依赖性的，并且被抗血管生成素单克隆抗体完全抑制，但不被非免疫对照抗体抑制。它不受非血管生成同源物RNA酶A的影响，也不受其他血管生成蛋白如碱性成纤维细胞生长因子及其抗体的影响。结果表明，在特定条件下，内皮细胞表达血管生成素受体，其可介导血管生成素刺激的DNA合成和增殖，并在血管生成素诱导的血管生成中起重要作用。
Angiogenin stimulates both [H-3]thymidine incorporation and proliferation of human endothelial cells in sparse cultures, Under these conditions, a 170-kDa cell surface protein can be detected that binds angiogenin specifically. Angiogenin-stimulated cell growth is concentration-dependent and is completely inhibited by an anti-angiogenin monoclonal antibody, but not by a nonimmune control antibody, It is not affected by the nonangiogenic homolog, RNase A, nor by other angiogenic proteins, such as basic fibroblast growth factor and its antibody, Results suggest that under specific conditions, endothelial cells express an angiogenin receptor that mag mediate angiogenin-stimulated DNA synthesis and proliferation and play an important role in angiogenin-induced angiogenesis.