IN-VIVO DETECTION OF SUPEROXIDE ANION PRODUCTION BY THE BRAIN USING A CYTOCHROME-C ELECTRODE
IN-VIVO DETECTION OF SUPEROXIDE ANION PRODUCTION BY THE BRAIN USING A CYTOCHROME-C ELECTRODE
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DOI：
10.1038/jcbfm.1995.30
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发表时间：
1995-03-01
影响因子：
6.3
通讯作者：
KENT, TA
中科院分区：
文献类型：
--
作者：
FABIAN, RH;DEWITT, DS;KENT, TA
采用细胞色素c包被的镀铂碳电极检测大鼠在缺氧/高碳、局灶性缺血、再灌注以及伴和不伴出血性低血压和再灌注的液体冲击脑损伤时脑产生的超氧化物。这三种形式的脑损伤都与超氧化物信号的增加有关。细胞色素c电极被证明具有足够的灵敏度和响应性，可以逐分钟测量脑组织产生的超氧化物。
A cytochrome c-coated platinized carbon electrode was utilized to detect superoxide generated by the brain during hypoxia/hypercarbia, focal ischemia, and reperfusion and following fluid percussion brain injury with and without hemorrhagic hypotension and reperfusion in the rat. All three of these forms of brain injury were associated with an increase in the superoxide signal. The cytochrome c electrode proved to be sensitive and responsive enough for minute-by-minute measurement of superoxide generation by brain tissue.