Prevalence of serum antibodies to hantaviruses in Northern Sweden as measured by recombinant nucleocapsid proteins
Prevalence of serum antibodies to hantaviruses in Northern Sweden as measured by recombinant nucleocapsid proteins
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DOI：
10.3109/00365549709011829
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发表时间：
1997-01-01
影响因子：
--
通讯作者：
Elgh, F
中科院分区：
文献类型：
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作者：
Ahlm, C;Juto, P;Elgh, F
基于汉滩、首尔、Dobrava、Sin Nombre和Puumala汉坦病毒重组核衣壳蛋白（rN Delta）（aa 1-117）的酶联免疫吸附试验（ELISA）用于确定瑞典北方随机和分层个体中抗体的流行率。总的来说，137/1533人（8.9%）有特异性血清免疫球蛋白抗体普马拉病毒，唯一的汉坦病毒已知发生在该地区。通过使用免疫荧光测定法，在相同的血清材料中，确定普马拉病毒抗体的流行率（8.9%）高于先前报道的（5.4%）。正如预期的那样，对Puumala病毒rN Delta反应的血清经常与Sin Nombre病毒蛋白交叉反应。出乎意料的是，21/1533（1.4%）的人只识别Sin Nombre病毒rN Delta。另外8名受试者在ELISA中对汉滩、首尔或多布拉瓦病毒衍生的rN Delta显示出反应性，但对普马拉病毒或辛农布尔病毒rN Delta没有反应性。目前在某些个体中发现的特异性识别Sin Nombre、Dobrava、Hantaan和首尔病毒蛋白的抗体证明，该地区可能存在抗原性不同于普马拉病毒的汉坦病毒。
An enzyme-linked immunosorbent assay (ELISA) based on recombinant nucleocapsid protein (rN Delta) (aa 1-117) of Hantaan, Seoul, Dobrava, Sin Nombre and Puumala hantaviruses nas used to determine the prevalence of antibodies among randomized and stratified individuals from northern Sweden. In total, 137/1533 individuals (8.9%) had specific serum Ige antibodies to Puumala virus, the only hantavirus known to occur in the region. The prevalence of antibodies to Puumala virus (8.9%) was determined to be higher than previously reported (5.4%) in the same serum material, by use of immunofluorescence assay. As expected, sera reactive to Puumala virus rN Delta did frequently cross-react with Sin Nombre virus protein. Unexpectedly, 21/1533 (1.4%) individuals recognized the Sin Nombre virus rN Delta exclusively. Another 8 subjects showed reactivity in the ELISA to Hantaan, Seoul, or Dobrava virus-derived rN Delta but not to Puumala virus or Sin Nombre virus rN Delta. The present demonstration in some individuals of antibodies specifically recognizing the Sin Nombre, Dobrava, Hantaan and Seoul virus protein justifies an awareness of the possibility that hantaviruses antigenically different from Puumala virus might occur in the region.