Investigation of approaches for the fabrication of protein patterns by scanning probe lithography
Investigation of approaches for the fabrication of protein patterns by scanning probe lithography
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DOI：
10.1021/la0100744
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发表时间：
2001-06-26
期刊：
影响因子：
3.9
通讯作者：
Porter, MD
中科院分区：
文献类型：
--
作者：
Kenseth, JR;Harnisch, JA;Porter, MD
本文研究了三种不同的方法来图案化蛋白质内的抗蚀剂层形成的自组装单分子层，使用扫描探针光刻（SPL）在亚微米的长度尺度。第一种方法使用“纳米接枝”方法将反应性羧酸封端的硫醇图案化到由甲基封端的单层组成的抗蚀剂中。兔IgG抗原与图案化区域结合，并使用扫描力显微镜进行免疫测定，利用直接读出抗兔IgG抗体特异性结合引起的地形变化。为了解决与非特异性蛋白质吸附相关的问题，另外两种方法研究了通过SPL在高尖端样品力下机械“刮擦”图案来图案化去除乙二醇封端的单层。通过二硫键偶联剂的化学吸附或通过Fab '-SH抗体片段的直接吸附实现蛋白质与刮擦区域的附着。从所有的方法获得的结果进行了介绍和比较，并对制造高密度，多个蛋白质阵列的优势和劣势进行了讨论。
This paper investigates three different approaches to patterning proteins within ultrathin resist layers formed from self-assembled monolayers using scanning probe lithography (SPL) at the submicrometer length scale. The first approach uses a "nanografting" method to pattern a reactive carboxylic acid terminated thiol into a resist composed of a methyl-terminated monolayer. Rabbit IgG antigen is bound to the patterned region, and an immunoassay utilizing direct readout of the topographic change resulting from specific binding of anti-rabbit IgG antibody is performed using scanning force microscopy. To address issues related to nonspecific protein adsorption, the other two approaches investigated the patterned removal of glycol-terminated monolayers by mechanically "scraping" patterns at high tip-sample forces by SPL. Protein attachment to the scraped regions was achieved either through the chemisorption of a disulfide coupling agent or by the direct adsorption of Fab'-SH antibody fragments. Results obtained from all approaches are presented and compared, and the strengths and weaknesses of each toward fabricating high-density, multiple protein arrays are discussed.