Inverted-colloidal-crystal hydrogel matrices as three-dimensional cell scaffolds
Inverted-colloidal-crystal hydrogel matrices as three-dimensional cell scaffolds
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DOI：
10.1002/adfm.200400325
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发表时间：
2005-05-01
影响因子：
19
通讯作者：
Kotov, NA
中科院分区：
文献类型：
--
作者：
Zhang, YJ;Wang, SP;Kotov, NA
功能性组织的成功工程化需要开发能够为组织生长和再生提供最佳微环境的三维（3D）支架。一类具有高度组织性和独特形貌的新型3D支架由聚丙烯酰胺水凝胶制成。水凝胶基质由直径为1041μm的聚甲基丙烯酸甲酯球体制成的反相胶体晶体模制而成。支架的形貌可描述为由通道网络相互连接的六边形堆积的97μm球形空腔。空腔的长程有序尺度超过几毫米。与块状的类似材料相比，呈反蛋白石形状的水凝胶表现出高得多的溶胀率；其溶胀动力学也快一个数量级。这种工程化支架具有理想的机械和光学性能，既能促进组织再生，又能对支架内的细胞增殖和细胞 - 细胞相互作用进行连续的高分辨率光学监测。对于接种在支架上的两种不同的人类细胞系，观察了支架的生物相容性以及细胞在支架内的生长和浸润情况，并在长达五周的时间内对支架内深度达250μm的区域进行了显微镜跟踪。易于生产、独特的3D结构、生物相容性和光学透明性使这种新型水凝胶支架适用于组织工程中极具挑战性的任务。
Successful engineering of functional tissues requires the development of three-dimensional (3D) scaffolds that can provide an optimum microenvironment for tissue growth and regeneration. A new class of 3D scaffolds with a high degree of organization and unique topography is fabricated from polyacrylamide hydrogel. The hydrogel matrix is molded by inverted colloidal crystals made from 1041 mu m poly(methyl methacrylate) spheres. The topography of the scaffold can be described as hexagonally packed 97 mu m spherical cavities interconnected by a network of channels. The scale of the long-range ordering of the cavities exceeds several millimeters. In contrast to analogous material in the bulk, hydrogel shaped as an inverted opal exhibits much higher swelling ratios; its swelling kinetics is an order of magnitude faster as well. The engineered scaffold possesses desirable mechanical and optical properties that can facilitate tissue regeneration while allowing for continuous high-resolution optical monitoring of cell proliferation and cell-cell interaction within the scaffold. The scaffold biocompatibility as well as cellular growth and infiltration within the scaffold were observed for two distinct human cell lines which were seeded on the scaffold and were tracked microscopically up to a depth of 250 mu m within the scaffold for a duration of up to five weeks. Ease of production, a unique 3D structure, biocompatibility, and optical transparency make this new type of hydrogel scaffold suitable for most challenging tasks in tissue engineering.