GENOMIC IN-SITU HYBRIDIZATION IN AVENA-SATIVA
GENOMIC IN-SITU HYBRIDIZATION IN AVENA-SATIVA
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DOI：
10.1139/g94-086
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发表时间：
1994-08-01
期刊：
影响因子：
3.1
通讯作者：
ARMSTRONG, K
中科院分区：
文献类型：
--
作者：
CHEN, QF;ARMSTRONG, K
用基因组荧光原位杂交技术研究了六倍体燕麦的基因组结构和进化。Sun II，AACCDD，2n=6x=42)。以普通燕麦(Avena Strigosa)(基因组组成A(S)A(S)，2n=14)和普通燕麦(Avena Pilosa，基因组组成CPCP，2n=14)的基因组DNA为探针进行了研究。六倍体燕麦42条(2/3)染色体中有28条(2/3)被粗毛燕麦DNA标记，而42条(1/3)染色体中有14条(1/3)标记有粗毛燕麦DNA，表明A、D两个基因组之间有较近的亲缘关系。结果还表明，A和C基因组之间至少有18条染色体(9对)参与了基因组间的互换。
Genomic fluorescent in situ hybridization was employed in the study of the genome organization and evolution of hexaploid oat (Avena sativa L. cv. Sun II, AACCDD, 2n = 6x = 42). Genomic DNAs from two diploid oat species, Avena strigosa (genomic constitution A(s)A(s), 2n = 14) and Avena pilosa (genomic constitution CpCp, 2n = 14), were used as probes in the study. The DNA from A. strigosa labelled 28 of the 42 (2/3) chromosomes of the hexaploid oat, while 14 of the 42 (1/3) chromosomes were labelled with A. pilosa DNA, indicating a close relationship between the A and D genomes. Results also suggested that at least 18 chromosomes (9 pairs) were involved in intergenomic interchanges between the A and C genomes.