Polyphosphate kinases modulate Campylobacter jejuni outer membrane constituents and alter its capacity to invade and survive in intestinal epithelial cells in vitro.
Polyphosphate kinases modulate Campylobacter jejuni outer membrane constituents and alter its capacity to invade and survive in intestinal epithelial cells in vitro.
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DOI：
10.1038/emi.2015.77
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发表时间：
2015-12-30
影响因子：
13.2
通讯作者：
Rajashekara G
中科院分区：
文献类型：
--
作者：
Pina-Mimbela R;Madrid JA;Kumar A;Torrelles JB;Rajashekara G
空肠弯曲杆菌是世界上最常见的细菌性肠胃炎的原因。多磷酸激酶1和2 （PPK1和PPK2）调节多种细胞过程，包括细菌细胞壁的生物合成。尽管它们很重要，但PPK1和PPK2是否调节空肠梭菌外膜成分（OMCs）的组成，从而影响其与宿主细胞的相互作用仍不清楚。我们对野生型、Δppk1和Δppk2菌株的比较分析显示，这些菌株的总OMC组成在定性和定量上存在差异。重要的是，在空肠梭菌多磷酸激酶突变体上观察到的这些OMC变异与它们在体外入侵、存活和改变肠上皮细胞免疫反应的能力直接相关。具体而言，空肠c OMC的亚分离表明，OMC蛋白与细菌侵袭有关，而空肠c OMC蛋白、脂质和脂聚糖都与空肠c细胞内存活有关。该研究为多磷酸激酶的功能及其在空肠梭菌感染中的作用提供了新的见解。
Campylobacter jejuni is the most prevalent cause of bacterial gastroenteritis worldwide. Polyphosphate kinases 1 and 2 (PPK1 and PPK2) regulate several cellular processes, including the biosynthesis of the bacterial cell wall. Despite their importance, whether PPK1 and PPK2 modulate the composition of C. jejuni outer membrane constituents (OMCs) and consequently impact its interaction with host cells remains unknown. Our comparative analysis between C. jejuni wild type, Δppk1, and Δppk2 strains showed qualitative and quantitative differences in the total OMC composition among these strains. Importantly, these OMC variations observed on the C. jejuni polyphosphate kinase mutants are directly related to their capacity to invade, survive, and alter the immune response of intestinal epithelial cells in vitro. Specifically, sub-fractionation of the C. jejuni OMC indicated that OMC proteins are uniquely associated with bacterial invasion, whereas C. jejuni OMC proteins, lipids, and lipoglycans are all associated with C. jejuni intracellular survival. This study provides new insights regarding the function of polyphosphate kinases and their role in C. jejuni infection.