[Determination of tramadol and its active metabolite O-desmethyltramadol in plasma and amniotic fluid using LC/MS/MS].
[Determination of tramadol and its active metabolite O-desmethyltramadol in plasma and amniotic fluid using LC/MS/MS].
复制标题
LC/MS/MS法测定血浆和羊水中曲马多及其活性代谢物O-去甲基曲马多[J].
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
D. Zhong
中科院分区：
文献类型：
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作者：
Li;Xiaoyan Chen;Jian Cui;Maleku Sunita;D. Zhong
目的
采用LC/MS/MS法测定人血浆和羊水中曲马多和O-去甲基曲马多的浓度，研究曲马多和O-去甲基曲马多在孕妇和胎儿体内的分布。
方法
样品中含有曲马多，O-去甲基曲马多和苯海拉明（内标，IS）提取液-液萃取，然后通过液相色谱分离和在线MS/MS使用大气压化学电离作为接口检测。在选定的反应监测模式下检测分析物。
结果
血浆和羊水中曲马多和O-去甲基曲马多的校准曲线在8.0 - 800.0 μ g × L（-1）（血浆）和1.0 - 400.0 μ g × L（-1）（羊水）范围内呈线性。方法应用于孕妇静脉、脐静脉、脐动脉及羊水中曲马多和去甲基曲马多的浓度测定。在术前肌内注射1.5 mg x kg（-1）剂量的曲马多给产妇后，血浆中的曲马多浓度显著高于羊水中的浓度。血浆中O-去甲基曲马多浓度较低，羊水中未检出。
结论
该方法准确、可靠、简便，适用于曲马多和O-去甲基曲马多的临床药代动力学研究。
AIM
To determinate tramadol and O-desmethyltramadol in human plasma and amniotic fluid by LC/MS/MS, and distribution of tramadol and O-desmethyltramadol in maternity and fetus were studied.
METHODS
Samples containing tramadol, O-desmethyltramadol and diphenhydramine (internal standard, IS ) were extracted using liquid-liquid extraction, followed by liquid chromatographic separation and on-line MS/MS using atmospheric pressure chemical ionization as an interface detection. The analytes were detected in the selected reaction monitoring mode.
RESULTS
The calibration curves for tramadol and O-desmethytramadol in plasma and amniotic fluid were linear in the range from 8.0 to 800.0 microg x L(-1) (plasma) and 1.0 to 400.0 microg x L(-1) (amniotic fluid). The method was applied to the measurement of tramadol and O-desmethytramadol concentrations in maternal vein, umbilical vein, umbilical artery and amniotic fluid. Following intramuscular pre-operative administration 1.5 mg x kg(-1) doses of tramadol to parturients, plasma concentrations of tramadol were significantly higher than those in amniotic fluid. The concentrations of O-desmethyltramadol in plasma were lower, and were not detected in amniotic fluid.
CONCLUSION
The method is shown to be accurate, robust and convenient, and suitable for clinical pharmacokinetics studies of tramadol and O-desmethyltramadol.