MDG-1 inhibits H2O2-induced apoptosis and inflammation in human umbilical vein endothelial cells
MDG-1 inhibits H2O2-induced apoptosis and inflammation in human umbilical vein endothelial cells
复制标题
MDG-1抑制H2O2诱导的人脐静脉内皮细胞凋亡和炎症
DOI：
10.3892/mmr.2017.6957
复制
发表时间：
2017-09-01
影响因子：
3.4
通讯作者：
Ruan, Yong-Le
中科院分区：
文献类型：
--
作者：
Li, Luo-Cheng;Wang, Zhi-Wei;Ruan, Yong-Le
MDG-1是一种从麦冬中提取的水溶性多糖，已报道其通过保护心肌细胞免受缺氧/再氧合诱导的损伤而起到抗心肌缺血的作用。然而，MDG-1是否能保护人脐静脉内皮细胞（HUVECs）免受氧化应激诱导的损伤仍不清楚。在本研究中，HUVECs用过氧化氢（H2 O2）处理，以建立氧化应激诱导的细胞损伤模型。不同浓度H2 O2处理HUVECs可显著降低细胞活力，并以时间和剂量依赖性方式增加细胞凋亡。MDG-1预处理显著降低H2 O2诱导的细胞死亡、ROS产生和炎性因子分泌。此外，与单独H2 O2处理相比，MDG-1预处理降低了促凋亡蛋白BCL 2相关X（Bax）和caspase-3的表达水平，而增加了抗凋亡蛋白BCL 2凋亡调节因子（Bcl-2）的表达水平。总之，目前的数据表明，MDG-1保护HUVECs对H2 O2诱导的凋亡和炎症通过抑制Bax/Bcl-2蛋白的比例，caspase-3的表达，和炎症因子的分泌。这项研究为MDG-1在心血管疾病治疗中提供了潜在的应用。
MDG-1, a water-soluble polysaccharide extracted from Ophiopogon japonicus, has been reported to serve a role in antimyocardial ischemia by protecting cardiomyocytes from hypoxia/reoxygenation-induced damage. However, it remains unknown whether MDG-1 protects human umbilical vein endothelial cells (HUVECs) against oxidative stress-induced damage. In the present study, HUVECs were treated with hydrogen peroxide (H2O2) to establish an oxidative stress-induced cell injury model. Treatment of HUVECs with different concentrations of H2O2 significantly attenuated cell viability and increased cell apoptosis in a time and dose-dependent manner. Pretreatment with MDG-1 markedly reduced H2O2-induced cell death, ROS generation and inflammatory factor secretion. In addition, pretreatment with MDG-1 decreased the expression levels of proapoptotic proteins BCL2 associated X (Bax) and caspase-3, while it increased the expression levels of the antiapoptotic protein BCL2 apoptosis regulator (Bcl-2), compared with H2O2 treatment alone. Taken together, the present data suggest that MDG-1 protected HUVECs against H2O2-induced apoptosis and inflammation through inhibition of Bax/Bcl-2 protein ratio, caspase-3 expression, and inflammatory factor secretion. This study provides a potential application for MDG-1 in the treatment of cardiovascular disease.