Effect of Candida antarctica lipase B immobilization on the porous structure of the carrier.
Effect of Candida antarctica lipase B immobilization on the porous structure of the carrier.
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DOI：
10.1002/mabi.201100127
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发表时间：
2011-11
影响因子：
4.6
通讯作者：
N. Miletić;Z. Vukovic;A. Nastasović;K. Loos
中科院分区：
文献类型：
--
作者：
N. Miletić;Z. Vukovic;A. Nastasović;K. Loos
研究了一系列组成相同但颗粒大小、孔径、比表面积和比体积不同的聚(GMA-co-EGDMA)树脂，以考察固定化假丝酵母脂肪酶B对共聚颗粒孔隙率的影响。汞测孔法显示，环氧树脂的平均孔径(高达6.1倍)、比表面积(高达3.2倍)和比体积(高达2.1倍)发生了显著变化。戊二醛改性的环氧树脂也有类似的行为。研究了树脂孔道性质对固定化过程中南极假丝酵母脂肪酶B的负载量和固定化脂肪酶的水解性(对-硝基苯乙酸乙酯水解性)的影响。
A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.