Effect of basic amino acids on susceptibility to carbapenems in clinical Pseudomonas aeruginosa isolates.
Effect of basic amino acids on susceptibility to carbapenems in clinical Pseudomonas aeruginosa isolates.
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碱性氨基酸对临床铜绿假单胞菌菌株对碳青霉烯类药物敏感性的影响。
DOI：
10.1078/1438-4221-00256
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
M. Maekawa
中科院分区：
文献类型：
--
作者：
Hideaki Muramatsu;T. Horii;Motoki Morita;H. Hashimoto;T. Kanno;M. Maekawa
我们评估了培养基组成（包括碱性氨基酸含量和pH）对铜绿假单胞菌临床分离株对亚胺培南、帕尼培南和美罗培南等碳青霉烯类药物敏感性的影响。培养基中的碱性氨基酸降低了对碳青霉烯类的敏感性，而对头孢他啶和氨曲南的敏感性则没有降低。在碳青霉烯类中，在1:16的muer - hinton琼脂（MHA）中加入碱性氨基酸后，对帕尼培南的敏感性急剧降低。175株临床分离株中，174株碳青霉烯类药物的mic受培养基组成的影响程度不同。其中一株碳青霉烯类的mic在MHA和1:16 MHA之间没有差异，但显示出孔蛋白（OprD）的产生减少。我们的研究结果表明，基于MHA测定的其他碳青霉烯类药物的mic，很难评估对单个碳青霉烯类药物的敏感性，尤其是帕尼培南。为了更好地预测抗生素的疗效，单独评估每种碳青霉烯类药物的敏感性可能很重要。
We evaluated effects of medium composition, including basic amino acid content and pH, on susceptibility to carbapenems such as imipenem, panipenem and meropenem, in clinical isolates of Pseudomonas aeruginosa. Susceptibility to carbapenems was reduced by basic amino acids in the medium, while susceptibilities to ceftazidime and aztreonam were not. Among carbapenems, susceptibility to panipenem was most sharply reduced by addition of basic amino acids to 1:16 Mueller-Hinton agar (MHA). In 174 of 175 clinical isolates, MICs for carbapenems were affected to different degrees by medium composition. One isolate, in which MICs for carbapenems did not differ between MHA and 1:16 MHA, showed reduced production of porin (OprD). Our results suggest that susceptibility to individual carbapenems, especially panipenem, is difficult to evaluate based on MICs for other carbapenems determined on MHA. For a better prediction of antibiotic efficacy, it may be important to evaluate the susceptibility for each carbapenem individually.
铜绿假单胞菌外膜的蛋白质 D2 通道具有碱性氨基酸和肽的结合位点。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Trias,J;Nikaido,H
通讯作者：
Nikaido,H