A new ascovirus from Spodoptera exigua and its relatedness to the isolate from Spodoptera frugiperda
A new ascovirus from Spodoptera exigua and its relatedness to the isolate from Spodoptera frugiperda
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DOI：
10.1099/0022-1317-81-12-3083
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发表时间：
2000-12-01
影响因子：
3.8
通讯作者：
Arif, BM
中科院分区：
文献类型：
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作者：
Cheng, XW;Carner, GR;Arif, BM
根据目前基于DNA限制性片段长度多态性（RFLP）、杂交、封闭体形成、组织趋向性和宿主谱的ICTV命名方案，从印度尼西亚斑点Spodoptera exigua ascovirus中分离到一种新的ascovirus，并初步确定为新种seev -5a。SeAV-5a主要在易感宿主的脂肪体中复制。SeAV-5a可传播给frugiperda、Pseudoplusia包括和Trichoplusia ni，但不能传播给Heliothis virescens。SeAV-5a感染后，宿主生长受到抑制，但存活时间延长，可达33天。特征性囊泡内可见成簇的病毒粒子。偶尔，病毒粒子被包含在液泡中（每个液泡1 - 5个），一些病毒粒子被包裹在闭塞体中。SeAV-5a病毒粒子大小为347 × 134 nm；但也可观察到异常的长二次病毒产物。阻断体的存在、Southern杂交和Western免疫印迹分析表明SeAV-5a与S， frugiperda ascovirus 1a（SfAV-1a）的亲缘关系比与毛癣ascovirus 2 （TnAV-2）的亲缘关系更密切。SeAV-5a基因组182 kb的部分区域与SfAV-1a存在杂交。SeAV-5a基因组DNA探针与SeAV-5a基因组DNA探针杂交。SeAV-5a DNA中有5 - 8个HindIII和EcoRI片段与SfAV-1a基因组探针杂交。
A new ascovirus was isolated from Spodoptera exigua in Indonesia and was tentatively assigned as a new species, Spodoptera exigua ascovirus 5a (SeAV-5a) according to the present ICTV ascovirus naming scheme based on DNA restriction fragment length polymorphism (RFLP), hybridization, formation of occlusion body, tissue tropism and host spectrum. SeAV-5a replicated primarily in the fat body of susceptible hosts. SeAV-5a could be transmitted to S. frugiperda, Pseudoplusia includens and Trichoplusia ni, but not to Heliothis virescens, Infection with SeAV-5a arrested growth of the hosts, but prolonged their survival, which continued up to 33 days. Clusters of virions were seen inside the characteristic vesicles. Occasionally, virions were contained within vacuoles (one to five per vacuole) and some virions were embedded in occlusion bodies. The size of the SeAV-5a virion was 347 x 134 nm; however, aberrant long secondary viral products were also seen. The presence of occlusion body and Southern hybridization and Western immunoblot analyses suggest that SeAV-5a is more closely related to S, frugiperda ascovirus 1a(SfAV-1a) than to Trichoplusia ni ascovirus 2 (TnAV-2). Certain regions of the 182 kb genome of SeAV-5a showed hybridization to that of SfAV-1a. Two fragments in each of the SfAV-1a EcoRI and HindIII digests hybridized to the SeAV-5a genomic DNA probe. Five to eight HindIII and EcoRI fragments in SeAV-5a DNA hybridized to the SfAV-1a genomic probe.