Red blood cell aggregation and microcirculation in rat cremaster muscle.
Red blood cell aggregation and microcirculation in rat cremaster muscle.
复制标题
大鼠提睾肌中的红细胞聚集和微循环。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
M. Duvelleroy
中科院分区：
文献类型：
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作者：
E. Vicaut;X. Hou;L. Decuypère;A. Taccoen;M. Duvelleroy
采用大鼠提睾肌活体显微镜，研究了红细胞聚集状态的改变对红细胞微动脉运动速度和毛细血管密度的影响。为了改变RBC聚集，通过等容血液稀释程序将2和/或10%葡聚糖（分子量40，000、70，000或480，000）或新鲜大鼠血浆输注至成年雄性大鼠中。高分子量葡聚糖（70，000和480，000）均诱导RBC超聚集，与RBC小动脉速度（2%和10%葡聚糖浓度分别为30%和40%）和PCD（两种浓度分别为35%和37%）的剂量依赖性降低相关。相反，用40，000分子量的葡聚糖或血浆，我们观察到RBC小动脉速度增加30%，但PCD或超聚集没有变化。在2%右旋糖酐70，000之前或之后静脉注射抗聚集药物曲塞鲁肽（10（-3）M），可显著抑制右旋糖酐对RBC小动脉流速和PCD的影响。我们的结论是，红细胞过度聚集可以导致小动脉的速度和PCD的变化，因此，可能会损害组织氧合。
Using intravital microscopy of the rat cremaster muscle, we studied the effects of changing red blood cell (RBC) aggregation on RBC arteriolar velocity and perfused capillary density (PCD). To modify RBC aggregation, 2 and/or 10% dextran (molecular weights 40,000, 70,000 or 480,000) or fresh rat plasma was infused into adult male rats via a normovolemic hemodilution procedure. The high-molecular-weight dextrans (70,000 and 480,000) both induced RBC hyperaggregation associated with similar dose-dependent decreases in RBC arteriolar velocity (30 and 40% for dextran concentrations of 2 and 10%, respectively) and in PCD (35 and 37%, respectively, for the two concentrations). Conversely, with 40,000 molecular weight dextran or plasma, we observed a 30% increase in RBC arteriolar velocity, but no change in PCD or hyperaggregation. Intravenous injection of the antiaggregating drug troxerutin (10(-3) M), either before or after 2% dextran 70,000, significantly inhibited the effects of this dextran on RBC arteriolar velocity and on PCD. We conclude that RBC hyperaggregation can lead to changes in both arteriolar velocity and PCD and may, therefore, impair tissue oxygenation.