A chemical screening method for menaquinone-producing strains based on HPLC-UV technology
A chemical screening method for menaquinone-producing strains based on HPLC-UV technology
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基于HPLC-UV技术的甲萘醌生产菌株的化学筛选方法
DOI：
10.1016/j.mimet.2020.105907
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发表时间：
2020-05-01
影响因子：
2.2
通讯作者：
Kuang, Bingdi
中科院分区：
文献类型：
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作者：
Cao, Sheng;Du, Xia;Kuang, Bingdi
尽管菜籽酮(MKs)-4和MKs-7具有广泛的生物活性和应用，但对其他MKs的研究较少。因此，为了获得一系列MK以进一步探索其药理活性、构效关系和应用，建立了一种基于高效液相色谱-紫外(HPLC-UV)技术的MK产生菌的化学筛选方法。考虑到芽孢杆菌已经被证明是一种重要的MK产生菌资源，我们使用了29个先前从发酵大豆样品中寻找的可能的芽孢杆菌菌株来验证化学筛选方法，最终发现了16个MK产生菌。其中，枯草芽孢杆菌DC-1菌株表现出较强的单核细胞产生能力。另一株购买的淀粉液化芽孢杆菌被发现是一株产MK的菌株。这些结果表明，这种筛选方法对于发现产生MK的菌株，特别是那些产生一系列MK结构的菌株，是一种简单而高效的方法。
Despite menaquinones (MKs)-4 and - 7 being known to have extensive biological activities and applications, less attention has been paid to the other MKs. Thus, to obtain a range of MKs to further explore their pharmacological activities, structure-activity relationships, and applications, a chemical screening method for MK-producing strains was established based on high-performance liquid chromatography-ultraviolet (HPLC-UV) technology. Considering that Bacillus strains have proven to be an important MK-producing bioresource, twenty-nine putative Bacillus isolates previously sought from a fermented soybean sample were used for the validation of the chemical screening method, which ultimately led to the discovery of sixteen MK-producing strains. Among them, Bacillus subtilis DC-1 presented excellent ability to produce MKs. Another, a purchased strain of B. amyloliquefaciens was discovered to be an MK-producing strain. These results indicated this screening method was simple and highly efficient for the discovery of MK-producing strains, especially those producing a range of MK structures.