Integrated in vivo neural imaging and interface CMOS devices:: Design, packaging, and implementation
Integrated in vivo neural imaging and interface CMOS devices:: Design, packaging, and implementation
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DOI：
10.1109/jsen.2007.912921
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Ohta, Jun
中科院分区：
文献类型：
--
作者：
Ng, David C.;Nakagawa, Takuma;Ohta, Jun
我们已经开发了两个CMOS设备，以展示使用CMOS技术的神经成像和接口的目的是在分子水平上研究大脑的功能。在这项工作中，我们讨论了一个紧凑的，单一的设备成像系统的设计，包装和实施的小鼠大脑内的成像。我们表明，该设备能够成像和测量荧光团浓度低至1 μ M。通过对小鼠海马体中丝氨酸蛋白酶的活性进行成像，对包装的装置进行体内荧光成像测试。结果表明，神经活动的空间分辨率接近7.5 μ m的像素大小和小于300毫秒的时间分辨率的成像。第二种设备被开发用于对神经元网络活动进行成像，并提供与神经元进行电接口的手段。表征测试表明，该设备具有与当前用于大脑电生理实验的工具相当的性能。这项工作铺平了道路，同时成像和电生理实验，在未来使用一个单一的紧凑和微创设备。
We have developed two CMOS devices to demonstrate the use of CMOS technology for neural imaging and interfacing with the aim of studying the functions of the brain at the molecular level. In this work, we discuss the design, packaging, and implementation of a compact, single device imaging system for imaging inside the mouse brain. We show that the device is capable of imaging and measuring fluorophore concentrations down to 1 mu M. The packaged device was tested for in vivo fluorescence imaging by imaging the activity of serine protease in the mouse hippocampus. The result shows imaging of neural activity with spatial resolution close to the pixel size of 7.5 mu m and less than 300 ms temporal resolution. A second device was developed to image neuronal network activity and to provide a means for electrical interfacing with neurons. Characterization tests show that the device has comparable performance to current tools used in electrophysiological experiments of the brain. This work paves the way for simultaneous imaging and electrophysiological experiments using a single compact and minimally invasive device in the future.