Cerebral blood volume affects blood-brain barrier integrity in an acute transient stroke model
Cerebral blood volume affects blood-brain barrier integrity in an acute transient stroke model
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DOI：
10.1038/jcbfm.2013.27
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发表时间：
2013-06-01
影响因子：
6.3
通讯作者：
Kim, Young Ro
中科院分区：
文献类型：
--
作者：
Huang, Shuning;Kim, Jeong Kon;Kim, Young Ro
缺血性卒中后血管储备不足可能诱导生化级联反应，随后恶化血脑屏障（BBB）功能。然而，脑血容量（CBV）恢复不良和血脑屏障破坏之间的直接关系尚未在急性卒中中进行研究。为了量化短暂性卒中急性期BBB的完整性，特别是对于未观察到标准造影剂（Gd-DTPA）外渗的病例，我们采用了水交换指数（WEI），这是一种新的磁共振图像衍生参数，用于估计BBB的水渗透性。表观扩散系数（ADC）和R-2弛豫速率常数也被测量用于概述组织异常，而分数CBV和WEI被量化用于评估血管改变。缺血皮质ADC值和R-2值的显著降低与CBV和WEI的变化无关。脑卒中小鼠分为两组：（1）高WEI组和低CBV组;（2）正常WEI组和正常CBV组。对于具有破坏的BBB的小鼠观察到的低CBV（特征在于高WEI）表明CBV恢复对于在急性中风中维持BBB稳定性的重要性。
Insufficient vascular reserve after an ischemic stroke may induce biochemical cascades that subsequently deteriorate the blood-brain barrier (BBB) function. However, the direct relationship between poor cerebral blood volume (CBV) restoration and BBB disruption has not been examined in acute stroke. To quantify BBB integrity at acute stages of transient stroke, in particular for cases in which extravasation of the standard contrast agent (Gd-DTPA) is not observed, we adopted the water exchange index (WEI), a novel magnetic resonance image-derived parameter to estimate the water permeability across the BBB. The apparent diffusion coefficient (ADC) and R-2 relaxation rate constant were also measured for outlining the tissue abnormality, while fractional CBV and WEI were quantified for assessing vascular alterations. The significantly decreased ADC and R-2 in the ischemic cortices did not correlate with the changes in CBV or WEI. In contrast, a strong negative correlation between the ipsilesional WEI and CBV was found, in which stroke mice were clustered into two groups: (1) high WEI and low CBV and (2) normal WEI and CBV. The low CBV observed for mice with a disrupted BBB, characterized by a high WEI, indicates the importance of CBV restoration for maintaining BBB stability in acute stroke.