Effects of high temperatures on UV-B/visible irradiation induced postharvest anthocyanin accumulation in ‘Yunhongli No. 1’ (Pyrus pyrifolia Nakai) pears
Effects of high temperatures on UV-B/visible irradiation induced postharvest anthocyanin accumulation in ‘Yunhongli No. 1’ (Pyrus pyrifolia Nakai) pears
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DOI：
10.1016/j.scienta.2011.10.025
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发表时间：
2012-02
影响因子：
4.3
通讯作者：
Dong Zhang;Bo Yu;J. Bai;Minjie Qian;Q. Shu;Jun Su;Yuanwen Teng
中科院分区：
文献类型：
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作者：
Dong Zhang;Bo Yu;J. Bai;Minjie Qian;Q. Shu;Jun Su;Yuanwen Teng
近年来，中国红沙梨（Pyrus Pyrifolia Nakai）的种植面积有所增加，因其具有吸引力的市场价值和营养价值而备受推崇。然而，水果着色不佳一直是一个值得注意的问题。采后紫外线 B (UV-B)/可见光照射已用于改善花青素的积累，从而改善苹果和其他水果的果皮着色。本研究以红沙梨‘云红梨1号’为模型，在高温（27°C）和低温（17°C）条件下评估UV-B/可见光照射的功效。结果表明，27°C 下的 UV-B/可见光照射比 17°C 下更能有效诱导果皮组织中花青素的合成并改善果实着色。 27°C 时的 PAL 活性明显高于 17°C 时的活性。 PyMYB10 和五个花青素结构基因 PpPAL、PpCHI、PpCHS、PpF3H 和 PpANS 在 27°C 辐照的果实中的表达也高于在 17°C 辐照的果实中。对于PpUFGT，转录分别在27℃和17℃开始照射后48小时和240小时达到最大值，但27℃时的峰值低于17℃时的峰值。 17℃和27℃照射温度下PpDFR的表达没有差异。这些结果表明，高温（27°C）增强的UV-B/可见光照射通过上调PyMYB10和花青素结构基因以及增加苯丙氨酸解氨酶的活性，诱导‘云红利1号’梨采后花青素的积累。
Red Chinese sand pears (Pyrus pyrifolia Nakai) have seen increased cultivation in China in recent years, prized for their attractive market value and nutritional benefits. However, poor fruit coloration has been a noticeable problem. Postharvest ultraviolet-B (UV-B)/visible irradiation has been used to improve anthocyanin accumulation and thus the coloration of fruit skin in apple and other fruits. In this study, the efficacy of UV-B/visible irradiation was evaluated under high- (27°C) and low-temperature (17°C) conditions using red Chinese sand pear ‘Yunhongli No. 1’ as a model. The results showed that UV-B/visible irradiation was more effective in inducing anthocyanin synthesis in peel tissues and improving fruit coloration at 27°C than at 17°C. PAL activity was markedly higher at 27°C than at 17°C. Expression of PyMYB10 and five anthocyanin structural genes, PpPAL, PpCHI, PpCHS, PpF3H, and PpANS, was also higher in fruit irradiated at 27°C than in fruit irradiated at 17°C. For PpUFGT, transcription reached a maximum at 48 and 240h after the onset of irratiation at 27°C and 17°C, respectively, but the peak value was lower at 27°C than at 17°C. There was no difference in expression of PpDFR between 17°C and 27°C irradiation temperatures. These results indicated that high temperatures (27°C) enhanced UV-B/visible irradiation induced postharvest anthocyanin accumulation in ‘Yunhongli No. 1’ pears by up-regulating PyMYB10 and anthocyanin structural genes and increasing the activity of phenylalanine ammonia-lyase.