In utero phenotyping of mouse embryonic vasculature with MRI
In utero phenotyping of mouse embryonic vasculature with MRI
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DOI：
10.1002/mrm.22991
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发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Turnbull, Daniel H.
中科院分区：
文献类型：
--
作者：
Berrios-Otero, Cesar A.;Nieman, Brian J.;Turnbull, Daniel H.
脉管系统是哺乳动物最早发育的器官，其正确形成对胚胎存活至关重要。MRI方法以前被用于分析固定小鼠胚胎中复杂的三维血管模式和缺陷。将血管成像扩展到具有纵向研究潜力的子宫内环境，将使正常和基因工程小鼠胚胎的血管系统动态分析成为可能。在这项研究中，我们采用了一种子宫内MRI方法来校正运动，使用交错门控采集和快速获取的三维图像的串行共配相结合。我们通过获取和分析野生型和Gli2突变胚胎的图像来测试这种方法的潜力，展示了大脑和脑血管系统中的许多Gli2表型。这些结果表明，子宫内MRI可用于多种突变小鼠胚胎的体内表型分析。医学杂志，2011。(C) 2011 Wiley-Liss, Inc。
The vasculature is the earliest developing organ in mammals and its proper formation is critical for embryonic survival. MRI approaches have been used previously to analyze complex three-dimensional vascular patterns and defects in fixed mouse embryos. Extending vascular imaging to an in utero setting with potential for longitudinal studies would enable dynamic analysis of the vasculature in normal and genetically engineered mouse embryos, in vivo. In this study, we employed an in utero MRI approach that corrects for motion, using a combination of interleaved gated acquisition and serial coregistration of rapidly acquired three-dimensional images. We tested the potential of this method by acquiring and analyzing images from wildtype and Gli2 mutant embryos, demonstrating a number of Gli2 phenotypes in the brain and cerebral vasculature. These results show that in utero MRI can be used for in vivo phenotype analysis of a variety of mutant mouse embryos. Magn Reson Med, 2011. (C) 2011 Wiley-Liss, Inc.